FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7207
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCT2833.9267378937144444Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCT921.2765367004301373Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCTC620.8602747328985707Illumina Single End PCR Primer 1 (96% over 31bp)
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG270.37463577077840987No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA270.37463577077840987No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCTC250.3468849729429721Illumina Single End PCR Primer 1 (96% over 30bp)
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC190.2636325794366588No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT160.22200638268350215No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA130.1803801859303455No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG130.1803801859303455No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT100.13875398917718884No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT100.13875398917718884No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA100.13875398917718884No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT100.13875398917718884No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT90.12487859025946996No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG80.11100319134175107No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG80.11100319134175107No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC80.11100319134175107No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT80.11100319134175107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)