FastQCFastQC Report
Sat 28 May 2022
EGAF00006158776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCAGCATCTCGTATG1626.730369754881595TruSeq Adapter, Index 8 (97% over 36bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC431.7864561695056087No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCAGCATCTCGTATGC331.3710012463647694TruSeq Adapter, Index 8 (97% over 35bp)
ATATATATATATATATATATATATATATATATATATATATATATATATAT291.2048192771084338No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT160.6647278770253428No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA130.540091400083091No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT130.540091400083091No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA130.540091400083091No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG120.4985459077690071No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG80.3323639385126714No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG80.3323639385126714No Hit
GGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCACAT70.29081844619858743No Hit
GCGGACTGGAGAGGCCTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGC60.24927295388450355No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC50.2077274615704196No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG50.2077274615704196No Hit
GCGCTGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG40.1661819692563357No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA40.1661819692563357No Hit
CGGGCACGGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGA40.1661819692563357No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA40.1661819692563357No Hit
GGTAATTCTATAACCAGTAATGTCTGGGGTGGTGCTCCTCTCCCAGGAGA40.1661819692563357No Hit
TTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACG40.1661819692563357No Hit
TGCAGAGATGGTCGGCGCGGCCTCACCGCGGTCAGGAGCAGCGCGAAACC40.1661819692563357No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA40.1661819692563357No Hit
GTCGGCATCATAGTTCTGTGTGGTCCCACACCACTTCATGTTGTCTCTTC40.1661819692563357No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC40.1661819692563357No Hit
GGGGTCTTGAGTGATAGGAAAGGGGTCTATGATGGGTAGATAGTGTGGTT30.12463647694225177No Hit
CTGGAAATTAAAAAAGAAAAGCTTTACTAAATATTGACATATATATTTAC30.12463647694225177No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT30.12463647694225177No Hit
GGGGCAGTAGTCTCTAGAACTTCTCCAGAAGGAAGCCCACTGATCTCATC30.12463647694225177No Hit
GCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATGATATTTGGCC30.12463647694225177No Hit
CTCACAAAGCTCTGCATTTCTGGACTGAGAGTTTTCACAAGTGTTGTTAG30.12463647694225177No Hit
CTCTGAAGCGTAATCGCATCTTAGGTCGGTATTTCTCCAAATAAAGCAGC30.12463647694225177No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC30.12463647694225177No Hit
GCTATGTTGGTGGAGGTAAACTGGCATCTAAGGTTTCTGTGCACATTTGT30.12463647694225177No Hit
ATTAATTCTAGCTTCGTTAATGGAGGATGCTGAAATCCAATTTCCATTTA30.12463647694225177No Hit
TGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGGCACTCTCTGCC30.12463647694225177No Hit
CAGGGGGTACTTGGAGACAGAGGGACCCACATTGTACTGCCTGGGCTGGC30.12463647694225177No Hit
GGGGGGGTGGGTGTGCGGAGGGAAGCGCGCGGCGGCGGCGGCGCCGCCGC30.12463647694225177No Hit
GTAGCCATCTGTGAAGCCAGTTACACTAAACTACTTCTACAGTTGATTGT30.12463647694225177No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCAGCATCTCGTATGCCG30.12463647694225177TruSeq Adapter, Index 8 (96% over 33bp)
GCCAAGGGAAAAACTCCACAATCCTAATAGATGGAGTTGTTTTCCTACTA30.12463647694225177No Hit
GGGCAAGTAAGAGGAATGCCGTTGATTTTGTCAGTTAGGGGCCGCGTGTG30.12463647694225177No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA30.12463647694225177No Hit
CGCCGGTCACGCCACTCGATGTCAAAGTAGCAGCTGGCATTCTTGCTGGA30.12463647694225177No Hit
TGAGGAATCATGTGGTCCAGGTCCCCGTTCTGCAGCTTCTCCATCACCCC30.12463647694225177No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA30.12463647694225177No Hit
TTAACAATTCCATGTCCAAGATGTATCCTATCAATTATAACAAAGGTATT30.12463647694225177No Hit
CAAAGAATAAAAGAAATCATGAACACCGCTTATTCAGCCACACAGTGCTT30.12463647694225177No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT30.12463647694225177No Hit
GGGGTGGGGAGGGAGCGAGCGGCGCGCGCGGGGTGGGGCGGGGGAGGGCC30.12463647694225177No Hit
GCCTTCACTTAAGCTCTCTCTCCCCGTATCCGGCGAGCCAACTGGATGTC30.12463647694225177No Hit
GTCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC30.12463647694225177No Hit
CCGGGGTTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG30.12463647694225177No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG30.12463647694225177No Hit
CGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAG30.12463647694225177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)