FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCT1144.736186123805567Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCT371.5371832156211052Illumina Single End PCR Primer 1 (96% over 31bp)
ATATATATATATATATATATATATATATATATATATATATATATATATAT351.4540922309929374No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG190.7893643539675945No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCTC180.7478188616535106Illumina Single End PCR Primer 1 (96% over 31bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA120.4985459077690071No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA90.3739094308267553No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA90.3739094308267553No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC90.3739094308267553No Hit
GGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCCCCC80.3323639385126714No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT80.3323639385126714No Hit
GAGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTC80.3323639385126714No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT80.3323639385126714No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC80.3323639385126714No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG80.3323639385126714No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA70.29081844619858743No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT60.24927295388450355No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCTC60.24927295388450355Illumina Single End PCR Primer 1 (96% over 30bp)
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG50.2077274615704196No Hit
GGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCC50.2077274615704196No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT50.2077274615704196No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG40.1661819692563357No Hit
GGGAGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCG40.1661819692563357No Hit
AAGACCAGAGCCCCGCAGCCCCTGGGGCCCCCCGGAGCCATGGGGTGTCG40.1661819692563357No Hit
GCCCGGCTGAGAGAGACGGGGAGGGCGGCGCCGCCGCCGCCCGCGAAGAC40.1661819692563357No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT40.1661819692563357No Hit
GCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCG40.1661819692563357No Hit
GCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCCC40.1661819692563357No Hit
ATGGACACAGCCATTATGCCTCTGAGTCGCTTCCCTCCAAGAAGGACCAG30.12463647694225177No Hit
CGGGCCCAGATAACAGGATACCGACTGACCGTGGGCCTTACCCGAAGAGG30.12463647694225177No Hit
TAAATAAAAGCTAGATACTGGAAACCTAACTGCAATGTGGATGTTTTACC30.12463647694225177No Hit
AGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGA30.12463647694225177No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCGTAGTGTAGATCT30.12463647694225177Illumina Single End PCR Primer 1 (96% over 31bp)
CAGAGACTCTCCGAGGCGGCGGCAGAGACAGAAGAGCGGGGTCGGGGCCG30.12463647694225177No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG30.12463647694225177No Hit
AGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACTCGAGACGTAA30.12463647694225177No Hit
CTTATGATAAAAACTAGAGCTAGTGAATTAGCCTATTTGTAAATACCTTT30.12463647694225177No Hit
ATCATGCCAGATTAAAGACAGACTGACAGTTCATCTCCTCACGGACTCCA30.12463647694225177No Hit
GACCACCCTCAACACGGATATCAGCATCCTGTCCTTGCAGGCTTCTGAAT30.12463647694225177No Hit
GCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCT30.12463647694225177No Hit
AGAGACTGCTGCCCCTGGAGTAGAGGACATCAGCGGGCTTCCTTCTGGAG30.12463647694225177No Hit
GCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGTTCCTCCCCT30.12463647694225177No Hit
CTTCCAGCTGGAGATCGACCGCGACACCAAAAAGTGTGCCTTCCGTACCC30.12463647694225177No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT30.12463647694225177No Hit
GAACTTGTTACAAGAATTCACAAGGTCCGACAGATGCATTTGCTAAAAAG30.12463647694225177No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG30.12463647694225177No Hit
CGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGA30.12463647694225177No Hit
CCACGTTCTAGATTGGCCATTTGATGATGGAGCTCCACCCCCTAATCAGA30.12463647694225177No Hit
CGTCTCAGCTGCCAGCTCACTCCAGGGCCTCCGGAGGCACATGGCAGGGG30.12463647694225177No Hit
CATGAGGCGGCAGGCAGCCCTGGGGGTCAAGAGGAAGTAACTGAAGGCCG30.12463647694225177No Hit
ACACATATGTATGTGTAATTGCTCTGTATCAAAAAGAAGAAAGAGGGTAA30.12463647694225177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)