FastQCFastQC Report
Sat 28 May 2022
EGAF00006158778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT41713.694581280788176TruSeq Adapter, Index 13 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1224.006568144499179TruSeq Adapter, Index 13 (97% over 39bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC260.8538587848932676No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA250.8210180623973727TruSeq Adapter, Index 13 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT170.5582922824302134No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA160.5254515599343186No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT90.2955665024630542No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT80.2627257799671593No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA70.22988505747126436No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC70.22988505747126436No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC70.22988505747126436No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG60.19704433497536944No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA50.16420361247947454No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG50.16420361247947454No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT50.16420361247947454No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG50.16420361247947454No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG50.16420361247947454No Hit
GCCCAGAATTGATTTCCTTTAAAAATGAAAACGAGGTCCTTGCTAGTAAC40.13136288998357964No Hit
TTCCCATTTGCCTTCTGTCATGATTGCAAGACCTCCCCAGCCATGTGGAA40.13136288998357964No Hit
CTGCTGTTAGACATTTAGGTTGCTTCCAAATCTTAGCTATTGTAAACAGT40.13136288998357964No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG40.13136288998357964No Hit
GGTACATCAAAGCTTCAGTGTTGGCTGAGTGAAAGAGACCCAGGGAGTGG40.13136288998357964No Hit
CGATCACCGGGGCAGGAGACTAGCGCCTTCTGAGACTATCCCCGATCACC40.13136288998357964No Hit
GTAGCTTCTGACATCTGGCTTGATGGTTCTCTGGATTGGAGTCTGGCCAT40.13136288998357964No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA40.13136288998357964No Hit
CTCGCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.13136288998357964No Hit
GGGGCAGTAGTCTCTAGAACTTCTCCAGAAGGAAGCCCGCTGATGTCCTC40.13136288998357964No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG40.13136288998357964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)