FastQCFastQC Report
Sat 28 May 2022
EGAF00006158779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCT2558.374384236453201Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCT1213.973727422003284Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCTC692.2660098522167487Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCTC210.6896551724137931Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATC190.6239737274220033Illumina Single End PCR Primer 1 (96% over 33bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT90.2955665024630542No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA90.2955665024630542No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT70.22988505747126436No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATC70.22988505747126436Illumina Single End PCR Primer 1 (96% over 32bp)
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC70.22988505747126436No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG60.19704433497536944No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT60.19704433497536944No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG60.19704433497536944No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA50.16420361247947454No Hit
AAGAAATCAACCTTGCTCCTGACAGCTCATCCGTGGTTGTATCAGGACTT50.16420361247947454No Hit
GAAAATATGCTCAATATCACTAATAACTGGGAAAATACAAATCAAAATCA40.13136288998357964No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTCGTCGCCTTAGTGTAGATCT40.13136288998357964Illumina Single End PCR Primer 1 (96% over 30bp)
AGAAGTTCTAGAGACCACTGCCCCTGGAGTAGAGGACATCAGCGGGCTTC40.13136288998357964No Hit
ATTGCCTTGGAGAAGAAAAAAGTCCAAGGATAATATGAAATTAAATCATT40.13136288998357964No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA40.13136288998357964No Hit
AGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCC40.13136288998357964No Hit
CAAGAAGGGCTCGTGTGACAGATGCTACTGAGACCACCATCACCATTAGC40.13136288998357964No Hit
TGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG40.13136288998357964No Hit
TGGACCTGGAAATGTTTTGGCCCATGCCTATGCCCCTGGGCCAGGGATTA40.13136288998357964No Hit
CAAGATCATGCCACTGCACTCCAGCCTGGGTGACAAAGTGTGACCCTGTC40.13136288998357964No Hit
AGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTG40.13136288998357964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)