FastQCFastQC Report
Sat 28 May 2022
EGAF00006158780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCGGATCTCGTATG481.5281757402101241TruSeq Adapter, Index 15 (97% over 38bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT220.7004138809296403No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC130.4138809296402419No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT90.28653295128939826No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCGGATCTCGTATGC80.2546959567016874TruSeq Adapter, Index 15 (97% over 37bp)
GGGGTGTTGAAGGTCTCAAACATGATCTGGGTCATCTTCTCGCGGTTGGC60.19102196752626552No Hit
TTAAAACAGAGTCTCTGAATCGACTTCACCTGAAAGGTGGAAGCCAGAGG50.1591849729385546No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA50.1591849729385546No Hit
CTCATATATTCATTCAACAATTAGTCATTCAGCACATTCTATGGTCCAGC50.1591849729385546No Hit
GGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGACGGA50.1591849729385546No Hit
GGCAGGGATGATGTTCTGGAGAGCCCCGCGGCCATCACGCCACAGTTTCC50.1591849729385546No Hit
GAAGAGCACACGTCTGAACTCCAGTCACATGGCGGATCTCGTATGCCGTC50.1591849729385546TruSeq Adapter, Index 15 (96% over 33bp)
GGACGGATCAGACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAGGGGGT40.1273479783508437No Hit
CCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGC40.1273479783508437No Hit
CTGGGGTTACCTGCCTGAGGATCACTTGTAGTGGGGGCTTAGGGGGCTGA40.1273479783508437No Hit
GCTTTAAGTTTTTTGTTATTTTTCTGTGCTGTGTACATTTGAATTTCCAA40.1273479783508437No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA40.1273479783508437No Hit
GTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACTTCTGAGGTAATAA40.1273479783508437No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC40.1273479783508437No Hit
GGCTGAAGGAGATGAGGAAATAAGGCCATATAGGAAGACGGGATGTCATT40.1273479783508437No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA40.1273479783508437No Hit
CCGAGAGGCCGTGAACTGGACCCCATAATCCATCTGCTCCCAGTGGGTTA40.1273479783508437No Hit
AAAGAGATATGATGAAACCACGGGACAGGAGGAGGGTGGGGTGGTGTGGA40.1273479783508437No Hit
CTTGCAGTGGTAGGTGATGTTCTGGGAGGCCTCGGTGGACATCAGGCGCA40.1273479783508437No Hit
GTTGAGCTGAAGCTGGAGAACTTCCTCCAGAGCAAAGGGCTTAAGAAAGA40.1273479783508437No Hit
CCCAAATCTGTAAGACATAATATTATAATTCAAATGCAACTCATGGCTTC40.1273479783508437No Hit
GCCGTGTCTGATTTCACATCAACAAGTCACTGGAAATTATGTCACATTAG40.1273479783508437No Hit
AGGTAGTTCACATTAAGACATGTAAGAACCAAGGTTTTTTGGTGTTTTTT40.1273479783508437No Hit
GGCAGGTATTAGGGATAATATTCATTTAGCCTTCTGAGCTTTCTGGGCAG40.1273479783508437No Hit
TGGAAGGGTTACCAGTTGGGGAAGCTCGTCTGTCTTTTTCCTTCCAATCA40.1273479783508437No Hit
CCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGATGC40.1273479783508437No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)