FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCGGATCTCGTATG1234.725316941990012TruSeq Adapter, Index 15 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCGGATCTCGTATGC250.9604302727621975TruSeq Adapter, Index 15 (97% over 37bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC200.768344218209758No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT130.4994237418363427No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA130.4994237418363427No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT90.3457548981943911No Hit
TGCTGGGTGCTGCTGGGGCTCTAGGGCTCTAGGGTATCTCACTTTCAGCA70.2689204763734153No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT70.2689204763734153No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG60.23050326546292738No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA50.1920860545524395No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT50.1920860545524395No Hit
GTGGAGATAAAGACTAAAGCAAGAGGCCTGAGGAACAGGCACGGAGAACG40.1536688436419516No Hit
TCCACATGATGGTGACCTTCACGTCTGTCACTTCCACAAACTGCAGGTCC40.1536688436419516No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG40.1536688436419516No Hit
CTCTGTTCTTCATCTGCTATTAACCTTGCCTTAGAAACACATAAATTATG40.1536688436419516No Hit
CCGGTGTATCGAAGGCAGTGGAGAGGAAGGGCCTGTGGGAGCCAGGGCCA40.1536688436419516No Hit
CGGTCCTGATAGCTTCCACCAGCTTAGCCAAAGCGCCTTTGTCTTCCGAG40.1536688436419516No Hit
GTTGTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAGGTTCG40.1536688436419516No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT40.1536688436419516No Hit
CCTGAGCTCAAGCAGTCTGCCCACCTCGGCCTCCCAAAGTACTGGGATTA40.1536688436419516No Hit
GTGCATAGGAGCTTCTGTTCAAGGTGTAAGACCTATCCGAACTGGCAGAA40.1536688436419516No Hit
GAAGAGCACACGTCTGAACTCCAGTCACATGGCGGATCTCGTATGCCGTC40.1536688436419516TruSeq Adapter, Index 15 (96% over 33bp)
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG40.1536688436419516No Hit
TGGGGGTGGAGCCCAGGTGACACGCATGGTGTCTGGACCAATGTTGGTGA40.1536688436419516No Hit
GACGTTTAGATTATAAGACATCAAACAATATAAAATCATCTAACTTAAGT40.1536688436419516No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG40.1536688436419516No Hit
CTTAAAATCAATCTATCCAGAAGACAAGAAAGACAAACTTAGTGAAGGAA30.11525163273146369No Hit
GGAATTTAAATGTTATTCATGCTTCTTATTTTAAAAGTGCTTCAAATCCT30.11525163273146369No Hit
GTCAGGATGTAAATGGCCAGTCTCAGGATAAAGATGGTAAGCTCCATTGC30.11525163273146369No Hit
GGCCAACTCAGCAGACATATCACAGGGCAGGAGAGGTGGGTTAAGGACAG30.11525163273146369No Hit
TTGGAAGATCCAGGTACTGTGGCTCATCTCCCTCCTCACTCAGCTCATAT30.11525163273146369No Hit
TGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCACATGGA30.11525163273146369No Hit
GGGCATAAGAAACCTTATACAATTTGCATAAACAGGTGAGAGTAAACGAG30.11525163273146369No Hit
GTCCTGAACTCCCGACTTCAGGTGGTCTGCCTGCCTTGGCCTCCCAGAGT30.11525163273146369No Hit
CGCAGACAGTGAGTTAGGGGATCAAATTCTATCCTAGTCACTAGTTTGGA30.11525163273146369No Hit
TTCAAAACTAAGGGAGTGAAGCTCTTCAGTAACGATGAGAGGACCCTCAT30.11525163273146369No Hit
AGAGAAAACAGGTTTAGAGAAATAGAAGCCTTAAACTTGTTTTTCATTCT30.11525163273146369No Hit
GGGGTCTTGGGCATGCATGGCAAGGATGCTGCGGGGGATGAGCTCTCGGT30.11525163273146369No Hit
GGCCAGTCTTACAAAATATAATGAAGGTTGAGACCCTACTGAGTACAGGT30.11525163273146369No Hit
GTTGTAAACCTGTGATGGTGTAGCTTCTGACATCTGGCTTGATGGTTCTC30.11525163273146369No Hit
TGCTGGTTTCATATTTTCTTTTTTATTCTTTGTTTGTGCCAAAAATGAAT30.11525163273146369No Hit
ATCAACAATGCACTGATCTCGAAGCTGCGAGTAGGCAATGCATGTCCATT30.11525163273146369No Hit
GTCCAGGGTGGCGTCAGAGAAGTAGAGCTTCTCGGCACGGTGGTCGATGG30.11525163273146369No Hit
GGGGCTTGGGCCTGCCTCCTCCAGGGTGGCCTCCAGGTGGGCTGTGGTGA30.11525163273146369No Hit
GTGAGGGTGATGGAATTCCGAGAGTGGGGCACCCGATCTTCTCGAGGTCT30.11525163273146369No Hit
CTATAAAACAACATCTTGCATGTAGTTAGAACCTAATAAATATTAGTATA30.11525163273146369No Hit
CCAGACTGACCAAATACAAAGTTGTCTGGTCTAAAGATCTGGCCAAAAGG30.11525163273146369No Hit
AGCGCAGCTACTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGG30.11525163273146369No Hit
GTCGGTTGTTAAGAACTAGAGCTTATTCCTATTCCAAATCTATCTTGCGC30.11525163273146369No Hit
ATGGATTTTCTGGAAGACGTGGATCTTCAGGAAGACATATATTGGCTGGA30.11525163273146369No Hit
GGCGGGCGGATCACGAGGTCATGAGATCGAGACCATCCTGGGTAACACGG30.11525163273146369No Hit
CTTGGAGAGGCAGAGCACGTTCGACTCTTCACTGACCGTCTCGCGGTACG30.11525163273146369No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCGGATCTCGTAT30.11525163273146369TruSeq Adapter, Index 15 (97% over 38bp)
GGTAATTCTATAACCAGTAATGTCTGGGGTGGTGCTCCTCTCCCAGGAGA30.11525163273146369No Hit
GATGTAGAAGGAGACGGCCCCGAACTTGCGCCACTTGTCCCGCAGCAGTT30.11525163273146369No Hit
GGTGGGTGCAGGCTCCTTGGTGGTGGGTGCAGGCTCCTTGGGAGTGGTGG30.11525163273146369No Hit
CAGTGGAGTTAAAATATGCCTGAATTAATGAAAATGGTTGGATTTAGGAA30.11525163273146369No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT30.11525163273146369No Hit
GCCGGCCTGGCTCAAGTTTTAAAAGCTGATTTAATAATTTTTTTTATGGA30.11525163273146369No Hit
CTGGAAAGAGGAATCTTATCTAAAGATACTGTAGTTACAAGGGCTGATAA30.11525163273146369No Hit
TTGCTCTATCGTGCTCTTTCCACTCCAGAACAGGGCTCATCACTGCCCGC30.11525163273146369No Hit
CCTGGTTGTAAACCTGTGATGGTGTAGCTTCTGACATCTGGCTTGATGGT30.11525163273146369No Hit
GCCACGACGAACATCCTTGACAGACACATTCTTGACATTGAAGCCCACAT30.11525163273146369No Hit
CAGGCTGCTCCCAATCTCAGCTCTGGAGGATTCTCCACTGACCTCAGCTA30.11525163273146369No Hit
TGGGAATTCTCTAATTGTATCATGTGGGCCTTTTGAAAGTAACAAACAGA30.11525163273146369No Hit
GCGGACTGGAGAGGCCTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGC30.11525163273146369No Hit
GCTGATTCATTTATCTTAAAACAATGGGAAACCCCAGCTGCAGACCTCAA30.11525163273146369No Hit
GGGGGATGGCCTACTGCCCTCCTTTCTGTACAATCTGGGCAAACCGACTG30.11525163273146369No Hit
CGGGATTTATGGGGATTTTTACTTTTTCTGGAATAGTAGAAATAATTTAT30.11525163273146369No Hit
TAAACAGCTTAGCGGAGTTCTTGATTACATACAGAAATGAAACCCCTGCA30.11525163273146369No Hit
GCTGCCATTACTGTTAATATTTGGATGATAAATTCTTGTTGTAAATGCAA30.11525163273146369No Hit
GTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGCGCTCCCAT30.11525163273146369No Hit
TGGGGGTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTG30.11525163273146369No Hit
GGACAGGATCAAACTCTGCTCCCCATCCTCAGATATCTGATAGACATTTA30.11525163273146369No Hit
CTGTTGATTTATCTGATAGTGTTTTCCATTGTCATAACAACCGGGCTTGC30.11525163273146369No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)