FastQCFastQC Report
Sat 28 May 2022
EGAF00006158783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCT782.996542451018056Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCT391.498271225509028Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCTC210.8067614291202458Illumina Single End PCR Primer 1 (96% over 31bp)
ATATATATATATATATATATATATATATATATATATATATATATATATAT100.384172109104879No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA90.3457548981943911No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA80.3073376872839032No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG70.2689204763734153No Hit
CGCAGATACACGCCCCCGCCTCCGTGGGCACAAAGGCAGCGCTGCTGGGG70.2689204763734153No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC70.2689204763734153No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCTC60.23050326546292738Illumina Single End PCR Primer 1 (96% over 30bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT50.1920860545524395No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT50.1920860545524395No Hit
CCCCCAGTGGCTATACAGCCTCTTTTCTATGGTAGCTCTAATAAGACTCC40.1536688436419516No Hit
AACGAAGAGACCACCTGTCCTTCGAGCAGGAGTTAACACCGTCACCACCT40.1536688436419516No Hit
CCCAGCTGTGCTTTTTCGCCATGTGGGAGACTTCAAGCCTGCAGCGTGGA40.1536688436419516No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC40.1536688436419516No Hit
GCGATAGACAAGGGTCCGGTCACTAGCCACAGAAAGGCTGTCACTGTTAT40.1536688436419516No Hit
GACAAACATAGGGATTAAAAGTTACAGGAATGCAGAAGTGAATGGGATGG40.1536688436419516No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC40.1536688436419516No Hit
ATCAGTAGAAGGTAGCAGCACAGAACTCAACCTTCCTGAAACTGCAAACT40.1536688436419516No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG40.1536688436419516No Hit
CAAGCTCATCGCAGATGCCTGGAATGCCATCCCCGATAACCTGGATGCCG40.1536688436419516No Hit
CAAGGAGCCTGGCCTGTCCCTACTGTTTTGTTTCTTCTACTTGTGTCACT30.11525163273146369No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG30.11525163273146369No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG30.11525163273146369No Hit
GCCTTAGTTTCCTTAGGTGTAATAATAATAATAATCCTCATTGTTATCAA30.11525163273146369No Hit
GAGGAATTCAGCGGTGGTGAAGAGAGTGGATACAAACCAGGGATTGGTTT30.11525163273146369No Hit
AAGCACCCCAAGGAGGCATTCCACAGCAACTTTGTGTCCCTCGACTGTGA30.11525163273146369No Hit
CAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACTCCCAAGG30.11525163273146369No Hit
CAGTTCCTGGTGAAGACGGGCACCATCACCACCTTCGAGCACGCGCACAA30.11525163273146369No Hit
AATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATT30.11525163273146369No Hit
AAACAGTTAAGTACTGATGTCAACAGACAAATATTTCTGATCAGATAGTC30.11525163273146369No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA30.11525163273146369No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC30.11525163273146369No Hit
GCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGAACTTAGTGA30.11525163273146369No Hit
CTTGCACTGTCGCCTAGGCTGGAGTGCAGTGGCGGGATCTCGGCTCACTG30.11525163273146369No Hit
CAGCCCTGCATGCCAACGCACCCTCAGAGGCCGCTGGTCTTGAAGACAGG30.11525163273146369No Hit
GAAAACTAAGCTGAATCGACTGCTGCCAAACATCTATTAGGCAAAATTGG30.11525163273146369No Hit
GCGACCTGCAGCTGGACCGCATCTCTGTGTACTACAATGAAGCCACAGGT30.11525163273146369No Hit
CTCAGATGTTAAAATAAATGGTTTGGTGCTTTTTATAAAAAGATAATCTC30.11525163273146369No Hit
ATTACTTTTCATTATATATGTGTCACAGTGAAACAGCTTTTATGTGTCAT30.11525163273146369No Hit
CAGAGCTCCTGCACTTTTGATAACCTGAGTCCCGGCCTGGAGTACAATGT30.11525163273146369No Hit
CAACATCAGGAGTCCCTGACCTGGTTTCTGGTACCACGAGTGGCAGCGGT30.11525163273146369No Hit
TGCCACCTGGCAAAGGACCCTTGAGTAAGTGAAGGTCTCCTAAAACTGGG30.11525163273146369No Hit
ATGAAGTGAGTGTCTATGCTCTTAAGGACACTTTGACAAGCAGACCAGCT30.11525163273146369No Hit
CTGAGGGCAGAAGAGACAACATGAAGTGGTGTGGGACCACACAGAACTAT30.11525163273146369No Hit
GAACTTCTTGAACAACTCTCCCCACTATTATGCATAATATTTCTGCACAA30.11525163273146369No Hit
TGCAGGGGTCTGGGGTTGGCAACTCCTGAGGCCTGCATGGGTGACTTCAC30.11525163273146369No Hit
GGAAAACGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTATCAACT30.11525163273146369No Hit
GCCGATGAAGCGGAGTTGCAGCGCCTGGTGGCCGCCGAGCAGCAGAAGGC30.11525163273146369No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT30.11525163273146369No Hit
AGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACT30.11525163273146369No Hit
CCAAAACTAACCCTAAACCCTACCTCTCCCTGTCTCCTGAAAATCTTTCA30.11525163273146369No Hit
CGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATC30.11525163273146369No Hit
CGGAGAGGTGGCATGATTGTGGATCCCCTGAGATCTGGCTTCCCAAGAGC30.11525163273146369No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT30.11525163273146369No Hit
CACAACCCCTACAAACGGCCAGCAGGGAAATTCTTTGGAAGAAGTGGTCC30.11525163273146369No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCTCGGTG30.11525163273146369Illumina Single End PCR Primer 1 (96% over 27bp)
CAGCGGTTGAACTCAGAGGAGAAAACTAAATAAGTAGAGAAAGTTTTAAA30.11525163273146369No Hit
TGTTCCTAAACACCCATCCTGATAATCAGTGTTCTGAGAATAATAGGGAG30.11525163273146369No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA30.11525163273146369No Hit
CAGCTTCCGACACCGTGTCGGGATTCCGGGTGGAATATGAGCTGAGTGAG30.11525163273146369No Hit
CGATCCCAACCGCCGAGGGCTCATCGAAAATCCTGCCTTGCTTCGTTGGG30.11525163273146369No Hit
CCAAATCCAAGTCTTCCAGTAAATCTAGTTCTTCCAGAAAAATCTAGATC30.11525163273146369No Hit
GTGTCAAGTGGCTGCCTTCAAGTTCCCCTGTTACTGGTTACAGAGTAACC30.11525163273146369No Hit
GTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAG30.11525163273146369No Hit
ATTCCTCGCTTTATGACCTCTCCTCCCTGGACACGTGTGGGGAAGAGGCC30.11525163273146369No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)