FastQCFastQC Report
Sat 28 May 2022
EGAF00006158786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5373
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT4177.761027359017309TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG831.5447608412432532TruSeq Adapter, Index 20 (97% over 43bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC641.191140889633352No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA340.6327935976177183No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT230.4280662572119859No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT210.3908431044109436No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA170.3163967988088591No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT140.2605620696072957No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC130.24195049320677461No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA100.18611576400521124No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT90.16750418760469013No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG90.16750418760469013No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT80.148892611204169No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG80.148892611204169No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG70.13028103480364786No Hit
AAAGACTCCAGTGGCTTTGGGGCTCTCTTGGTTGCCCTTTATGGCCACGA70.13028103480364786No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG70.13028103480364786No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG70.13028103480364786No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA60.11166945840312675No Hit
CTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCT60.11166945840312675No Hit
ATTAAAAAAAACAAAAAGTAGAAACTCAATTCTTTTGATTCAGTGCTCTT60.11166945840312675No Hit
GTGGTAATTCTATAACCAGTAATGTCTGGGGTGGTGCTCCTCTCCCAGGA60.11166945840312675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)