FastQCFastQC Report
Sat 28 May 2022
EGAF00006158788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3508
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAACCATCTCGTATG1052.993158494868871TruSeq Adapter, Index 6 (97% over 38bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT280.798175598631699No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA220.6271379703534777No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAACCATCTCGTATGC170.4846066134549601TruSeq Adapter, Index 6 (97% over 37bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC110.31356898517673887No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT110.31356898517673887No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC100.28506271379703535No Hit
TGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTT60.17103762827822122No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT60.17103762827822122No Hit
GGCATTACCAAATACTCGTACTACCTAAGCACTGTGGCTGTGTACCACAC60.17103762827822122No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA50.14253135689851767No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA50.14253135689851767No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT50.14253135689851767No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG50.14253135689851767No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG50.14253135689851767No Hit
CTGCAAGTATAGCTGCTTTTTGAGAAATTTAGTTGAAAACAATAGGAAAG40.11402508551881414No Hit
GGGGCGGGAAAAAGGGGTGCAGCCAGACAAGAGAATATACAGGAAAGAAG40.11402508551881414No Hit
GTACAAATTCAGAGGTTTAAAAAACTTCGAAAGTCACAGACACAGAATTT40.11402508551881414No Hit
GAGGTAAGGCGACTGGGGCCATTAGGGACAGCCCTCCAGGTGGGCCCATG40.11402508551881414No Hit
CGGACAGGGCTATTTCCTCCTGTTTCTCCGTAAGTGATCCTGTAATATCT40.11402508551881414No Hit
AATTTAACAGAAATGTTTAGTTTAATGGCATAATTGAAAAACAACCAACC40.11402508551881414No Hit
GGGGTAGCACAGTACGTCTCCAGGAGGGCCAGGTCACAGCTGCGGAAACA40.11402508551881414No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT40.11402508551881414No Hit
TTCCGGTGCAGAACGGCCTGGTCCTGGGGCGTCGCGTCCAGACCCAGGGC40.11402508551881414No Hit
GGCAGAAATAAAAACTAGTGATACTGATAACATCTAGACCTTTGCAGACG40.11402508551881414No Hit
TGGAAAAACATCCCTTTTTTAAATTTCACACAGCAAAGCATGTTAAAAAC40.11402508551881414No Hit
CGGATGTAGGAGAGCTTCTTCATTCCCTGGAAAGCCCCATTTTCAATTCC40.11402508551881414No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)