FastQCFastQC Report
Sat 28 May 2022
EGAF00006158789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3508
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCT782.2234891676168758Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCT260.7411630558722919Illumina Single End PCR Primer 1 (96% over 31bp)
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA130.37058152793614596No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCCTTAGTGTAGATCTC120.34207525655644244Illumina Single End PCR Primer 1 (96% over 31bp)
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG100.28506271379703535No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT100.28506271379703535No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT80.22805017103762829No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT80.22805017103762829No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA70.19954389965792474No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA50.14253135689851767No Hit
AGAGGAGATGTCATCCATGCTCCTGAGGGTCTCCCTGTAGAGTTGCAGCT50.14253135689851767No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT50.14253135689851767No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG40.11402508551881414No Hit
CCTCACTCCAGGCACAGAGTATGTGGTCAGCATCGTTGCTCTTAATGGCA40.11402508551881414No Hit
CTAGATTTGGATCATTGCCCAAAGTTGCACTTGCTGGTCTCTTGGGATTT40.11402508551881414No Hit
TAGACACTTTTAGAATTTATTAAATCCTTGACCTTGTGCATTATAGCATT40.11402508551881414No Hit
CTCCGCGATGACTGCCAGCTCAGTGGAGCAGCTGCGGAAGGAGGGCAATG40.11402508551881414No Hit
CCTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTGTTGCCATGTTGGC40.11402508551881414No Hit
CCAGAATTCCCAAACCATGTAACACTCATTGAGAATTCCAGATCCATTAT40.11402508551881414No Hit
AGAAAACAGGTCTTGATTCCCCAACTGGCATTGACTTTTCTGATATTACT40.11402508551881414No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC40.11402508551881414No Hit
TTGATCTCTTGCCCTTCTACCTGTGCTGGAATCCTACTCCGTCAATTATC40.11402508551881414No Hit
TTTCCTGATCATAGCAAGAAATGCAGTGTCTGCCTGTTACAGCAAAAGAA40.11402508551881414No Hit
TGCGAACGCTACGCCATGGTTTATGGATACAATGCTGCCTATAATCGCTA40.11402508551881414No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTCGCCTTAGTGTAGATCTC40.11402508551881414Illumina Single End PCR Primer 1 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)