FastQCFastQC Report
Sat 28 May 2022
EGAF00006158795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12808332
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCACTCGAGTGTAGATCT146585511.444542505612752Illumina Single End PCR Primer 1 (96% over 32bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1828401.427508281328123No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT800020.6246090435507138No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCACTCGAGTGTAGATCTC469670.36669099458071513Illumina Single End PCR Primer 1 (96% over 31bp)
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT406890.3176760252623058No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT322140.2515081589078109No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA296480.23147432468177748No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG285410.2228315131119337No Hit
GTGAGGGTGATGGAATTCCGAGAGTGGGGCACCCGATCTTCTCGAGGTCT188980.1475445826981999No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT173480.13544308501684685No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA149260.11653351896250035No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA142000.11086533359691175No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG134730.10518934081346423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)