FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCAGCGTCTGGCCCAGGATGCTGTCACAGGCAGGAACAGGTTGGCAGA650.0No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT216.666666666666664TruSeq Adapter, Index 13 (97% over 40bp)
GGCCGGCCGGCGCACGCGTCACACCGGCCCGAACCCACCGCGATCGCTCA18.333333333333332No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAGAATCTCGTAT18.333333333333332TruSeq Adapter, Index 13 (97% over 39bp)
CTGAGGTGAAGATTCCAATTACATTCGAGACTTAAGTTCTTTCAATTTTT18.333333333333332No Hit
GGAGGTCATATTCATATCAATACCCCAGGCTTTTCAAATAGCATCTTTTC18.333333333333332No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)