FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGCGATCTCGTATG861.53846153846154TruSeq Adapter, Index 8 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCCTTGCGATCTCGTATGCCGTC17.6923076923076925TruSeq Adapter, Index 8 (96% over 33bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGCGATCTCGTAT17.6923076923076925TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACCCTTGCGATCTCGTATG17.6923076923076925TruSeq Adapter, Index 16 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGCGATCTCGTATGC17.6923076923076925TruSeq Adapter, Index 8 (97% over 37bp)
CAGGGATGTTCACAGAAGTGGCTGTGCTTGGAAGATCCAGGTACTGTGGC17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)