FastQCFastQC Report
Sat 28 May 2022
EGAF00006158801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA430.76923076923077Illumina RNA PCR Primer (96% over 31bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGAGGGAAAGAGTGTCGCTTAGTGTA17.6923076923076925Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAAAGAGTGTAGATCTCGGTGGTCGCCGTATC17.6923076923076925Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGGGAAAGAGTGTCGCCTT17.6923076923076925Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGAGTGTAGATCTCGGTGGTCGCCGTATCA17.6923076923076925Illumina Single End PCR Primer 1 (100% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTTCGCCTTAGTGTAGATCTCG17.6923076923076925Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCGTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA17.6923076923076925Illumina Single End PCR Primer 1 (96% over 30bp)
CTGTGACCGAAATCACAGCCAGTAGCTTTGTGGTCTCCTGGGTCTCAGCT17.6923076923076925No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTTCGCCTTAGTGTAGATCTCGG17.6923076923076925Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGGAAAGAGTGTCGCCTT17.6923076923076925Illumina Single End PCR Primer 1 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)