FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006165593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006165593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16250193
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT600670.36963868675282807TruSeq Adapter, Index 19 (97% over 38bp)
AGTGCCGATATTCCTCTTGAAATTTAAGCGTAACGAATTGTGTTACGCAT542370.33376218977829986No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTAT324510.19969608976336464TruSeq Adapter, Index 19 (97% over 38bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC255020.15693352072803074No Hit
GTGCCGATATTCCTCTTGAAATTTAAGCGTAACGAATTGTGTTACGCATA253360.1559119943990819No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG234450.14427520953135756No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA209150.1287061636744868No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA198440.12211547272084707No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT175920.10825717577631232No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT173850.10698334475165927No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)