FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006165603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006165603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959435
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT5886204.216646304094686TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTAT3269742.342315430388121TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTT209450.15004188923119022TruSeq Adapter, Index 19 (97% over 38bp)
AAAGGTACACAAGGCACCCCATTTTAACATTTCCCAACAGGTTTTTAACA154060.11036263287160261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)