FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006165613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006165613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14600942
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1280440.8769571168764317TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTAT506420.34684063535078763TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTT405250.27755058543483013TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGAT274270.18784404458287693TruSeq Adapter, Index 27 (97% over 39bp)
ATACCAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCC198070.13565563098600078No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTT179770.12312219307493996TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTTT167130.11446521738117993TruSeq Adapter, Index 27 (97% over 39bp)
GTAGCCCTTATTTAGAATAAGTCAAAAAAAAAGTAAGGGACAAACATTCC152760.10462338662806825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)