FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006165641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006165641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11885281
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGCAGCCCCGAGGCGCCCTACTTAGATGGAGGGAAAGAGCGTAATGG559610.47084288541432046No Hit
CCTCCCGCCCTTTCGTCCCTTACGGTATCCTAAATGTCTACTATTCTCCC265090.22304058271739644No Hit
GATGGTGATGTTATTGTGTGTTATGTGCCATTTCTGGACAATCAGTCAGT262830.22113907109137765No Hit
CCGCCGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGT254910.21447536663205521No Hit
CTCCGCCGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCAC252860.21275054413942757No Hit
CTCTGACGTCATTTGTCTCAGGGCCCACGTTAAGCGAAAGGATGGAGCAT237460.19979334102407845No Hit
CCGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAA225130.1894191647635424No Hit
CGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAAGCGAAAGGA215680.1814681537609418No Hit
ATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAAGCGA213730.17982746895088134No Hit
CGCCGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTT211390.1778586471788088No Hit
CGTCATTTGTCTCAGGGCCCACGTTAAGCGAAAGGATGGAGCATACTGTA200400.1686119158646733No Hit
GCCGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTA200130.16838474412174184No Hit
GTGGATGGTGATGTTATTGTGTGTTATGTGCCATTTCTGGACAATCAGTC190400.16019814760795306No Hit
CTGTGACGTCATCGTCCCCTATTAGATGCGCCCGGGGCGGAGGTTTAGTA188540.15863318671220308No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT171880.1446158487965072TruSeq Adapter, Index 7 (97% over 36bp)
CTCTAAGTAGTGTCCACAGTCCCTCACCTCCCGCCCTTTCGTCCCTTACG168290.14159530599234466No Hit
TGGACAATCAGTCAGTCAGATTATTCGCACTTAAATTTGAATTTTTCTGA167870.14124192772556238No Hit
CCCGCCCTTTCGTCCCTTACGGTATCCTAAATGTCTACTATTCTCCCATA166970.14048468858245758No Hit
CTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAAGCGAAA166730.1402827581442963No Hit
CATTTCTGGACAATCAGTCAGTCAGATTATTCGCACTTAAATTTGAATTT163390.13747255954655174No Hit
CCTCCGCCGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCA154920.13034609783310971No Hit
CATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAAGCG153680.1293027905692764No Hit
ATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAAGCGAAAGGATGG152990.12872224055956272No Hit
TTCTCCCATAGTCACCGTGTGTCCAATATGTTTGCAAAAAAAAGATGAGA152530.12833520721975356No Hit
TCCGCCGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACG152210.12806596663553854No Hit
CCATTTCTGGACAATCAGTCAGTCAGATTATTCGCACTTAAATTTGAATT139210.11712806790180223No Hit
CGCATCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAAG134760.11338394102756173No Hit
GTCATTTGTCTCAGGGCCCACGTTAAGCGAAAGGATGGAGCATACTGTAC132970.11187787650960883No Hit
TCTAAGTAGTGTCCACAGTCCCTCACCTCCCGCCCTTTCGTCCCTTACGG132560.1115329120110833No Hit
TCTACGCATCTACTCTGACGTCATTTGTCTCAGGGCCCACGTTAAGCGAA132320.11133098157292201No Hit
GGATGGTGATGTTATTGTGTGTTATGTGCCATTTCTGGACAATCAGTCAG128610.1082094735496788No Hit
CCTAAATGTCTACTATTCTCCCATAGTCACCGTGTGTCCAATATGTTTGC123540.10394369304352165No Hit
GTCTACTATTCTCCCATAGTCACCGTGTGTCCAATATGTTTGCAAAAAAA123260.10370810753233348No Hit
TTTCTGGACAATCAGTCAGTCAGATTATTCGCACTTAAATTTGAATTTTT120920.10173928576026096No Hit
CCGAGGCGCCCTACTTAGATGGAGGGAAAGAGCGTAATGGCGCAGTTCCG120330.10124287343311446No Hit
ATCAGTCAGTCAGATTATTCGCACTTAAATTTGAATTTTTCTGAATTCAG119550.10058659950909028No Hit
ACGTCATTTGTCTCAGGGCCCACGTTAAGCGAAAGGATGGAGCATACTGT119420.10047722052175291No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)