FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006165645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006165645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22936188
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2144720.935081278545502TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTAT1152270.5023807792297482TruSeq Adapter, Index 19 (97% over 38bp)
GTTACGTTAATTTTAACATTTGAAAAATAAAGATAAGGGACAATGTTTTT596440.26004321206296355No Hit
ATACAACATACCACACTACTATATAGCTACTATACTCTAGAGCTACTTGG269580.11753478825688034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)