FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006166061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006166061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19979389
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTAT202172510.119053190265227TruSeq Adapter, Index 23 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGT562500.281540141192506TruSeq Adapter, Index 23 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCGCGTAT338120.16923440451557353TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCCCGTAT285610.14295231951287402TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCACGTAT252390.1263251844188028TruSeq Adapter, Index 23 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTAT236610.1184270449912157TruSeq Adapter, Index 16 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)