FastQCFastQC Report
Sat 9 Jul 2022
EGAF00006167170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006167170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236197363
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA74371823.14871508535851No Hit
AGCAGGAACAATGTCTTTTCAAGTCCTTTGCCCATTTTTAAATTGAATTT45422321.9230663468499436No Hit
AAATCATGACTGATATGGTAGACAGAGCTTTCTCAGACTACTGCGAGTAC42042351.7799669507741287No Hit
TCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAAGCATTGG32721911.3853630533546644No Hit
CAGCTTTTCATGCAACACCCAACACTTATAAGCGGAAGAACACAGAAACA25503011.0797330535819742No Hit
CAGCTGTATAGGTACTTGAAGTGCAGTTTCTACTAAATGCATGTTGCTTT24761751.0483499767099433No Hit
GGCAGAAATGGGCTTGAATAGTTAGATGCTTATTTAACCTTGGCAATAGC22707580.9613816052637302No Hit
TCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTA21137690.8949164263108221No Hit
TTGATTTATCTTCATTAAAGTTAAGGCGACATAAATCTAAATTACTAAAG20931010.8861661169350141No Hit
AGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA19406990.8216429579698568No Hit
AAAGTCTGTGTCATGTTTCTGTGAGGTGAACAGTTTAAAGGGACTACTGC18850110.798066064776515No Hit
CACGTAATCAGCAATGAGTATTCTCTTCATTTCAGGTCAGTTGATTTATT18436260.7805447006620476No Hit
TTCTTTGTTTACATTTTAGGCTGCTGGTCTGGCTACTATGATCTCTACCA17951960.7600406614192385No Hit
CACTAGACTTGTCTTCTAAAGTGGTGGATGTAGATGCTTCCAAAGCTGAC15177000.6425558612184845No Hit
TTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAATGACT14739290.6240243249455752No Hit
GTTTATGTCTAAAAGGAGGAGGGCAACTATTGGATCGACTACTGCGAGTA14601810.6182037688541002No Hit
TTGTCACTTGTAGAAACCTAGTTACCTGTTTGTCTGAATTTTATGTTCCC13952620.5907187033243889No Hit
TTCCTGGAATGGTAGCCATCTGTTCTCTCCTGCTAGGACTACTGCGAGTA12676630.5366965083348538No Hit
GAGGAGGATGGGGAGTAGGACATACCAGCTTAGATTTTAAGGTTTTTACT12127630.5134532344461441No Hit
AGACACCTAAGACCATGGCACCTTCTCCCCAGAGGACAGCCTGAGGGTAA11790340.49917322743353404No Hit
GCAACCCAGAACCGAGTTTCAAAGCAAACGAGAACGGCCTTCCACCAAGC10239870.4335302422491482No Hit
TTAGGCTGCTGGTCTGGCTACTATGATCTCTACCATGAGACCTGATATAG9570910.4052081648345922No Hit
CAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAG9179190.3886237290464585No Hit
TATGATAGGTGACTGTCCGGAGATTCAGAAAGCGGCCGGAGCCCAGTTCG9050880.3831914076026327No Hit
AGAACCCTGCATCTAAAGTGGCATGGTAAGTATGTAGACTACTGCGAGTA8226550.34829135666514616No Hit
GCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCACTTCTGTG7931930.335817889719624No Hit
TTGTGGACAGGTTTTGAAAGATATTTGTGTTACTAATGACTGTGCTATAA7923440.33545844455511553No Hit
TAGGACTCTGGGAAGGTGGTATGGTAAGTATGTAGACTACTGCGAGTACA7502590.3176407181141984No Hit
TTTGGTACACTGCCTTGTTTAGTCTCGGTAACTGTGTGGGAAGAACTTGT7162330.3032349688002232No Hit
AATCCTGTTGATTCCCACGCCTATCCTCACATCCAGAACAGTAAGCAGCC6970710.29512226180103457No Hit
CTTCGTGCCAGTGCCTATGCATCTGTACTGCCAGAAAGTCCCGGTGCCTT6810510.2883397982728537No Hit
CTCCTGTTAAATTATAGTTTACACTGACACATGACTCGACTACTGCGAGT6638790.28106960703028677No Hit
CCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTGTCTCCT6580070.2785835504861246No Hit
GGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTG6295450.26653345829267366No Hit
CGGAAGCTGAGGAAATGAGAGGGTGGTCTTGAGTGAATATTCAAACCTGT6290780.2663357422834564No Hit
GACACAGTCCACAAATGACCCTAGACAGGCAGCAGGGTCCCGGGCCATCC6289340.26627477631915814No Hit
GATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG6259770.2650228571772835No Hit
CAAAGACTGCTTTAAAATTTTCAATGGAGATCTTACTAAAAAAATGCAAA6209500.2628945522986216No Hit
TTGCAACTGTGAGCTGTTACTATGTAAGACATTCTACTGCCATCTGCAGC6208900.2628691498134973No Hit
TGGAAATTGTCACCATTGCAGAGGCAGAATTTTATCGGCAGGTTTCTGCA5981740.25325176894544754No Hit
ACTAAACATCATAGTGTGGCGGACACCCTTTGGAGGCATAGTATCTTGTT5947180.2517885858022894No Hit
TGGAGTTTCCTGTCAGCCTGTCTCTTATACACATCTGACGCTGCCGACGA5910850.2502504653280147No Hit
TAGGACTCTGGGAAGGTGGTATGGTAAGTATGTAGACTACTGCGAGTACG5836410.24709886367359654No Hit
CATGTATGTATGTGTGTCCCGAGAAGCGGTTTGATACTGAGCTGCATTTG5805110.24577370069961366No Hit
TAGGACTCTGGGAAGGTGGTATGGTAAGTATGTAGACTACTGCGAGTACC5682770.2405941339827744No Hit
TAGGACTCTGGGAAGGTGGTATGGTAAGTATGTAGACTACTGCGAGTACT5673580.24020505258562094No Hit
CCCAGCCCCTTTATTGCTATTATGCAGAGCCAAAAGGAGAAGACTAGTTC5607540.23740908572294264No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA5595950.2369183943852921No Hit
CTTTGCCTGTTGCTTTTAAAGGAGACAACTGCTTTATAAGCAAGTGTGGC5538180.23447255844257667No Hit
GGAGCCTTGCCTGTAGTTAAGGTGTGTTATAGAACCAGGGTTTGGGTGGT5463500.23131079579410885No Hit
AGTATTCTCTTCATTTCAGGTCAGTTGATTTATTATTACTATTTCTTAAC5454850.2309445766335672No Hit
TCAATTTGATTCTGCCTACCTCCCAATCTTCCTGGCACTTACTTGGACCT5406440.22889501945879048No Hit
TCTGAAACCGAAGCCATCTAAGCTGGCAAAGAGAATCGCCAACTCAGCGG5270670.22314686044991958No Hit
ACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAA5196950.22002574177765058No Hit
GATTCCTTCCTCTCTTATTTGCCCTCCATTAGAACCAGATGATGAAAGTG5078620.21501594833639187No Hit
TGTATCTGTTTTCTACTTTGCCCTACTTTCTTAGATTCAAGTATATAAAA4956270.20983595824480053No Hit
CCAGGGCAGAAGATTTGACAGTGTCATTATCAAGCTTGGCTTTCAACTTT4799110.20318220064124934No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT4698750.19893321162946262No Hit
TCTATCACTCACCAAATAAATGCTCATATAACACAGCTTCAGCTTCTTGC4641140.19649414968278034No Hit
GGTACTGGTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTC4557860.19296828474753125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4557530.1929543133807129No Hit
CCCAATGATGAGGACTTTGTGCCATTCCGGCCCCGGCGCTCTCCTCGCAT4547870.1925453333702121No Hit
CCCACCGACAAGAACCTAAAGCCGAACCCCAACTGGAATCAAGGGAAGAC4399950.18628277403757468No Hit
TAAATAAATAACCCTCAACCGAAACCCCACTTCATACTTTCACTTCTCCC4370100.1850190004026421No Hit
GCTGCTGGAACCTGAACAGACTCAACAGCATGCCCGACTACTGCGAGTAC4226100.17892240397281656No Hit
TTCTTTGTTTACATTTTAGGCTGCTGTTCTGGCTACTATGATCTCTACCA4179500.1769494776281647No Hit
TTTCGGTAACTCTGCGTGTGATCGATGTCAATGCCAATTTTTTCTTCAGT4040820.1710781165664411No Hit
GTGCTTTGATTGTGTCTATGATTTAGGGAGCAACTTTTTGTGGTCCTTAT3991650.16899638291050692No Hit
GCTACACTGCGCGTATAAGCCAATCGCTTCTCAAACCAAATAGCTGGATC3973630.1682334616072746No Hit
CCGGGCTCAACTATTCATATGGTGTCAATGCTTGGAAGTGCTGGGTGCAG3946740.1670950068989551No Hit
GGCAGCAGACCCCTAAATGATGCTGTCCCTGTCATCAGGCGCTTCGCACA3897480.16500946287025228No Hit
AGTCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATA3647280.15441662657343047No Hit
AGTCTTCCGTTTCACTCTCAGAAGCAGAGGATCTCTGGGCCTGCCTCTTA3596320.15225910883687555No Hit
GAACAAACAGGTCTGAGGATTTACAAGACATTCCAGACTACTGCGAGTAC3489800.14774932097781293No Hit
GGCCCCATATTGGTTATTAAAGTTTTAAATATCAGAAGTATTTTAATTTT3483140.14746735339293351No Hit
AGAAGCCTCTCCATACTGACCTGCTTCTTCTGACCAATTCTATGCGCTCG3449800.1460558219695281No Hit
CCCCTATTCATACCTTGGAACCCTTATCACCTGAAGACTACTGCGAGTAC3440270.14565234583080422No Hit
TACCATTTTGAAGATGAGTCAGAAAATTACCTGTTTCTGAACCTCCACCT3354360.1420151333357604No Hit
GGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC3301730.13978691201560958No Hit
GCTGATTTGGCGTGAACTTCTGTAATTCCGTTCTCGCTTGGTGCAGATAT3292310.1393880929991585No Hit
GTTGTCCCTATAAGTCTTCCCAACCCAGGGCCTTTGCTCCTTCTTAGATT3271770.1385184812584042No Hit
TGTATCATCTATGGCTTCTTTCACAGTCCAATAGCAGCGCAGAGCAGCTG3252570.13770560173442747No Hit
GGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTTCACA3201600.1355476606231205No Hit
TCAATGGTGCTAGTTATCTGCGGCTGCCCTGGGTCAATCCTTACATGGAG3185650.13487237789356693No Hit
CTCTTGCAGCAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGC3139960.13293797865135354No Hit
GGTATCTTATTCCTCTTATGGATGCAGAATATGCCAACTACTATACTAGA3131330.13257260624031608No Hit
TCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGA2988270.12651580703718526No Hit
TGATAGGTCACCAACAAGGAAATAGAGCATTCTGATGAAAAGCACCTAGA2841790.12031421366884608No Hit
CAGTTCCGTCTGTGTGTTCGAGTGGACAAAATGGCGAAGATCGCCAAGAC2820570.11941581244495096No Hit
TTTTCTTGGGTTTTGGCTCCTTTCGGATGATGGATATAAATTTGCAGTTG2807560.11886500189250632No Hit
TGTCACCACACCAAGATAAACTCATGTCTATGCCAGTTATGACTGTGGAT2786770.11798480578295026No Hit
TTTAGATCCTAGCAAGTTCTTTCCTGCACACATCACAGCAAAAAACAATG2781730.11777142490790636No Hit
GCTATTTCCTCTGGCTTCCTTGGTATCTGTTGGGCCGGCTGAGGGGTCAG2780880.1177354380539803No Hit
CTGTATCCCTCTCAGGCATAAGGTAATGTACTTAGGGTGAAACATAAGGT2760680.11688022105479645No Hit
ACTGAAATAGTGACAGTGTGCTCTTAAATTAGATTTAATTATTACACTTC2737650.11590518900077644No Hit
CTTGACCATTGTAGATACGGTGTTCTCTCGAGTGGAGGAGGAGCACCTCT2718340.11508765235452693No Hit
GTGGCATTCACTTCCTTATCCAGAGCCCAGGCCCAGCACACTGCCTCTGA2681440.11352539951938413No Hit
TTAAATGGAAATCTTCACGCTTACACCAAAATCGATTTCTATCATTTCAT2660790.11265113065635707No Hit
TTTTCCTCAGTTGCTCTTTCTCTCTCCTCTTCCCTTATTTCATCTTCTGC2660660.11264562678458014No Hit
TGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAA2580240.10924084702842343No Hit
CCAAATTTTATGACAAAAGTTGTGGACAGGTTTTGAAAGATATTTGTGTT2512200.10636020521533086No Hit
GGGGCAAATTCCTGTTCACCTGAGGGGCTACTAGAGCCTTTGGCTCCAGA2501110.1058906826152839No Hit
TTGGGTCTTGTTTCCTGCAAAAAGTTCAGGATGTGTAGTCTGTTCTTTAT2491380.10547873898151859No Hit
TTTCTTAATCCTGTCCCTGCACAACATATCACTGCAGCCAAACCCATGCG2480170.10500413588444676No Hit
ACATACAAGAGATTAAAATGACACTGAATGAGAAAATGGTAACACTTTAC2466990.10444612796121691No Hit
CTTCATAAAGGAACAGAGAAGAATCCGGAAATTTCTTTTACACAGTCCAG2464130.10432504278212454No Hit
TGTGCTGTATCACTTGTACTTACTGCTCCCAGACCTGCCAGCGCGGAGTC2434250.10305999902293574No Hit
ATCCAGGCTAAGATGATGTGTGCACGTACCGACGTTATAGGTATTGAGGT2407450.10192535468738488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)