FastQCFastQC Report
Fri 10 May 2024
EGAF00006167971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006167971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145270123
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACGCGCGGCCTTACAGGAACG5105090.3514205050958758No Hit
CAGACGCGCGGGAACCTCCTCATT4854960.334202236477765No Hit
CAGACGCGGTTTGTCGAAGCCAGT4168660.2869592118401387No Hit
CAGACGCGCTATCGGCTTGAGAAC3996120.2750820277064128No Hit
CAGACGCGGCGGTCAACTTGGATA3953850.27217227591939186No Hit
CAGACGCGCGCATTATGTAGCCTA3900070.26847020705007596No Hit
CAGACGCGTGACAAATGAACCTAT3758500.25872491344968435No Hit
CAGACGCGACTAAAGCTCATCACG3441860.236928277399476No Hit
CAGACGCGATAAGCCACCGTAAAT3377660.23250892408207022No Hit
CAGACGCGAGCAATACTTCGCTTT3376140.2324042914178575No Hit
CAGACGCGCGGCCTTACCTGCAAG3336170.22965286537273738No Hit
CAGACGCGTGGTCATTGCTTCTTA3280840.22584409872083605No Hit
CAGACGCGACATTAGGATCCGTCA3238850.22295362137195962No Hit
CAGACGCGTCGATTCACCAGTTGG3214040.22124576847780325No Hit
CAGACGCGGGGTAATTGTTCACTT3129200.2154056137200352No Hit
CAGACGCGGCTGGTTTGCATGAGG3032480.20874767208670983No Hit
CAGACGCGTTCTAAGCTCCTACTT2952470.20324000138693352No Hit
CAGACGCGGAAGCACCTTGATTTG2897840.19947942083039333No Hit
CAGACGCGCCTTACCTGTGACATC2850950.196251640813989No Hit
CAGACGCGCTGGTTTACCTGGAAC2797190.19255094869025477No Hit
CAGACGCGTGAAAGCCTTAATTCG2713070.18676035677342961No Hit
CAGACGCGCGGATTAGAGGAACCT2687060.18496989914436845No Hit
CAGACGCGTATGTCCACCTGCGAA2652860.18261566419958217No Hit
CAGACGCGGCACGCAACGGTTCAA2652270.18257505020492065No Hit
CAGACGCGCCACAAAGAGTCTTAC2562720.17641067186265133No Hit
CAGACGCGTCCTCAAACCACAATA2558050.17608920176931356No Hit
CAGACGCGCCACTTAACATTGCGA2547720.17537811267634157No Hit
CAGACGCGGAATATCGACTAGGTA2545840.17524869859165743No Hit
CAGACGCGGACTTGCTGCAAGCGA2537360.17466495846499697No Hit
CAGACGCGGGGCTATGAAATGCAT2536680.17461814911521759No Hit
CAGACGCGCGCAGGACTAGCCAGA2462880.16953795791857354No Hit
CAGACGCGTAGGTTGGAGTGTGAC2452800.16884407814537336No Hit
CAGACGCGCTGCCAGTGTCCCGGA2395320.16488731134343432No Hit
CAGACGCGGCACCTATGAGTAGCA2385630.16422027810907822No Hit
CAGACGCGCAAGCTCCTTAGCGGG2378680.16374185901942137No Hit
CAGACGCGTACAACCTGCGAGTTT2360420.16248489030328694No Hit
CAGACGCGTTTAGTGCTCTGTTGG2358620.16236098320092976No Hit
CAGACGCGTGTGTTACTTGTGTGC2355190.16212487133366024No Hit
CAGACGCGTGACTTACTTTGTGAT2340220.1610943772657231No Hit
CAGACGCGCTGCCAGTGGGTTAGA2305130.1586788771425491No Hit
CAGACGCGGAGAAGCACTAAAGCT2289400.15759606674250562No Hit
CAGACGCGCAGAACCACGTAAACC2264760.1558999161857941No Hit
CAGACGCGCAGGTCCTGCCTGCAG2257650.15541048313148326No Hit
CAGACGCGCTTATGACTCAAGGGC2250000.1548838779464653No Hit
CAGACGCGCTTGAATCTCGACAAA2236540.15395732816995No Hit
CAGACGCGACCCGGAACACGCGCA2202820.15163613511912563No Hit
CAGACGCGTGAGCTTTGCTATGTG2196910.15122930679971958No Hit
CAGACGCGCAAGGCTACCTACCGC2136010.14703711650330192No Hit
CAGACGCGGGAGGCTTGGTCAGTC2128200.14649949735362996No Hit
CAGACGCGGTCCTGATGCGCGCTA2127490.14645062288547797No Hit
CAGACGCGTCAATGAACCGCAAAC2114430.1455516080205976No Hit
CAGACGCGGTAACGCGAAACGATT2097450.14438275102169493No Hit
CAGACGCGAATTAGCGATTGTGGA2084680.1435036989677499No Hit
CAGACGCGATAAGCCACCTGATTC2069780.14247802350934885No Hit
CAGACGCGCATGAAATGCCTGCAG2058040.14166987385286375No Hit
CAGACGCGCGTTGCACTAGCTAAA2048820.14103519413967866No Hit
CAGACGCGCCCTAAGCTGGCTAGA2046870.1409009614454584No Hit
CAGACGCGTAGCTCATGCGAGTTT2045490.1408059660003179No Hit
CAGACGCGCGCAGGACTAGTTACC2041120.14050514709070633No Hit
CAGACGCGAGCTTGAACTCAACAA2001810.13779915364978387No Hit
CAGACGCGAAGCTAACTTATGTTG1994140.13727117171918413No Hit
CAGACGCGCGATTGAGAGCTAGAC1971120.1356865375545941No Hit
CAGACGCGTAGGTTTCTTTGTTGA1970520.13564523518714167No Hit
CAGACGCGAGGCCTTTGTTGTTCT1960660.13496649961534074No Hit
CAGACGCGTAGAGATTGTTTCGGA1944370.13384514033900832No Hit
CAGACGCGGCTTCGCGATACTTGT1942090.13368819134268922No Hit
CAGACGCGGACTCAAACGTACCTC1940820.13360076799824835No Hit
CAGACGCGCTAGTTTGAGTCCCTG1927470.13268179032243263No Hit
CAGACGCGAGGGACCTGCTCTCAC1923470.1324064412060834No Hit
CAGACGCGAGATTGACTCCATGAC1921600.1322777154941901No Hit
CAGACGCGTGAATTGACCCGGATA1909800.13146543560095975No Hit
CAGACGCGTACCCTTCTAGGATCG1888980.13003224345036177No Hit
CAGACGCGGGATTTAGATATTCGT1877890.1292688380252834No Hit
CAGACGCGGCATAATGAACGGTGT1868420.1286169489923265No Hit
CAGACGCGCTCATTGACGATTCTT1846560.12711216607147774No Hit
CAGACGCGGGAGGCTTGTTTGCAG1840570.12669983076974473No Hit
CAGACGCGCATTTGTGATAACTTC1834330.12627028614823985No Hit
CAGACGCGAAGCCCTTGACCGCTA1826700.12574505770880362No Hit
CAGACGCGGACACCTTGATTATGC1821320.12537471314731385No Hit
CAGACGCGGGTATAGTGGAGCATA1814110.12487839636509429No Hit
CAGACGCGCCGGTAAACGGATCGC1813930.12486600565485856No Hit
CAGACGCGGAGGAACACCTCGACC1809510.12456174488129264No Hit
CAGACGCGGCACAAATGCGAGTGA1782240.12268455228058148No Hit
CAGACGCGGACTAGGCTCTCACAG1780890.12259162195381358No Hit
CAGACGCGCTATTGCACAGGAAAT1775650.12223091461139603No Hit
CAGACGCGTCCGCACACCATAAAC1771790.121965202714119No Hit
CAGACGCGGCTATGTCTTAGGAGT1753100.12067863396797703No Hit
CAGACGCGGTGCGTGACTCACGGA1752890.12066417813936868No Hit
CAGACGCGCTCAAAGGACATAACG1745390.12014789854621381No Hit
CAGACGCGTGGCTATTGAGTTACT1744770.12010521943317967No Hit
CAGACGCGACTAAGGTGAGGCGCA1727740.11893292057032265No Hit
CAGACGCGGTGACCTTGGCGAAAT1709830.11770004490186878No Hit
CAGACGCGAATGAGGGAGGCGGAA1698570.11692493713934557No Hit
CAGACGCGGGGTTAATGGCTTGCA1687420.11615740147752197No Hit
CAGACGCGCGCAAGGCTAAATACG1685830.11604795020377315No Hit
CAGACGCGAGGCCCAACGTGCAAT1664130.11455418124757834No Hit
CAGACGCGAAAGTGAACCAAACTA1662730.1144578090568561No Hit
CAGACGCGTAACCGTTGTGGAGCA1649220.11352781741638643No Hit
CAGACGCGCCTTTATTGGAAACTG1634890.11254137920706517No Hit
CAGACGCGATATGCGACGGTAAGG1634010.11248080240146834No Hit
CAGACGCGCCAGGAATGGGACAAT1628920.11213042065091389No Hit
CAGACGCGCATAGTGGAGGAGGAC1618310.11140005711979743No Hit
CAGACGCGCTGGTTATGGCAATTT1613110.11104210326854339No Hit
CAGACGCGGTTCACTGAACGAATT1605280.1105031073732897No Hit
CAGACGCGACTCATGCTCACACAC1571520.10817916083130183No Hit
CAGACGCGTAGTCAAACCGCTAAG1549880.10668952211185229No Hit
CAGACGCGTGCTATTGAACCGCAA1523240.10485569699696612No Hit
CAGACGCGGATGAAGGAATTAGGT1521740.10475244107833516No Hit
CAGACGCGTAGGCATGACCGTTAC1520660.10467809681692083No Hit
CAGACGCGGCGGTGTACCCTTCTC1497690.10309690451628516No Hit
CAGACGCGTACCTCCTGATCAGGA1492550.10274308090177633No Hit
CAGACGCGACAGAATCTTAGCTAT1485980.10229081997817266No Hit
CAGACGCGCGTCAAGACCTCCTCT1472250.10134568413630379No Hit
CAGACGCGGTGATCCTGATTGATC1467850.10104280010831959No Hit
CAGACGCGCTATCGCGAGGAACGG1455300.10017889225577374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)