FastQCFastQC Report
Sun 21 May 2023
EGAF00006171452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006171452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA23815.197956577266922No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA21613.793103448275861No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1439.13154533844189No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1066.7688378033205625No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1026.513409961685824No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA734.6615581098339725No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA402.554278416347382No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA402.554278416347382No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA362.2988505747126435No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA311.979565772669221No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA281.7879948914431671No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA271.7241379310344827No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA191.2132822477650063No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA171.0855683269476373No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA171.0855683269476373No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA161.0217113665389528No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA100.6385696040868455No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA90.5747126436781609No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA90.5747126436781609No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTCTTACACCAAGTAT90.5747126436781609No Hit
TACAGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCGCGTA80.5108556832694764No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA70.4469987228607918No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGAGTA70.4469987228607918No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA70.4469987228607918No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA60.38314176245210724No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA60.38314176245210724No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA60.38314176245210724No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA60.38314176245210724No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA60.38314176245210724No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTCTTACACCAAGTAT50.31928480204342274No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTCTTACACCAAGTAT50.31928480204342274No Hit
TACGGAAGGGGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGCGTA50.31928480204342274No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC50.31928480204342274No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTCTTACACCAAGTAT40.2554278416347382No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA40.2554278416347382No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC40.2554278416347382No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCCCGTA40.2554278416347382No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC30.19157088122605362No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGCGCGCGCA30.19157088122605362No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTCTTACACCAAGTAT30.19157088122605362No Hit
TACGGAGGGAGCTAGCGTTGTTCAGAATTACTGGGCGTAAAGCGTACGTA30.19157088122605362No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC30.19157088122605362No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTCTTACACCAAGTAT30.19157088122605362No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA30.19157088122605362No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA30.19157088122605362No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA30.19157088122605362No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTCTTACACCAAGTAT30.19157088122605362No Hit
TACGTAGGGTGCGAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGCA30.19157088122605362No Hit
CATATTAGATACCCTAGTAGTCCGGCTGACTGACTCTTACACCAAGTATC30.19157088122605362No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA30.19157088122605362No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC30.19157088122605362No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC30.19157088122605362No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC30.19157088122605362No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTCTTACACCAAGTAT30.19157088122605362No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA30.19157088122605362No Hit
TACGTAGGGTCCAAGTGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA30.19157088122605362No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTCTTACACCAAGTAT30.19157088122605362No Hit
TACGTAGGGGGCAAGCATTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.1277139208173691No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA20.1277139208173691No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA20.1277139208173691No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA20.1277139208173691No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC20.1277139208173691No Hit
TACGTATGGTGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA20.1277139208173691No Hit
TACCGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA20.1277139208173691No Hit
CATATTAGAAACCCCTGTAGTCCGGCTGACTGACTCTTACACCAAGTATC20.1277139208173691No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTCTTACACCAAGTATCTC20.1277139208173691No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA20.1277139208173691No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTCTTACACCAAGTAT20.1277139208173691No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAATGGTGCGTA20.1277139208173691No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTCTTACACCAAGTAT20.1277139208173691No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGCTCGTA20.1277139208173691No Hit
TACGCAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.1277139208173691No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA20.1277139208173691No Hit
TACGTAGGTGACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA20.1277139208173691No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTCTTACACCAAGTAT20.1277139208173691No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA20.1277139208173691No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.1277139208173691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)