FastQCFastQC Report
Tue 30 May 2023
EGAF00006171453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006171453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA21913.984674329501914No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1247.918263090676884No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1187.535121328224776No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT905.747126436781609No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG815.172413793103448No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA623.959131545338442No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA452.8735632183908044No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG342.1711366538952745No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG261.6602809706257982No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGGCCAGTG201.277139208173691No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA201.277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA191.2132822477650063No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT191.2132822477650063No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC181.1494252873563218No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA181.1494252873563218No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA150.9578544061302682No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA140.8939974457215836No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA120.7662835249042145No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGCA100.6385696040868455No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC90.5747126436781609No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA80.5108556832694764No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA80.5108556832694764No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA70.4469987228607918No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT70.4469987228607918No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG70.4469987228607918No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGCGTCAGTTGCAGACCAGGC70.4469987228607918No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCCAGTCCAGA70.4469987228607918No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG60.38314176245210724No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG60.38314176245210724No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG60.38314176245210724No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG60.38314176245210724No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA60.38314176245210724No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA60.38314176245210724No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA60.38314176245210724No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA50.31928480204342274No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA50.31928480204342274No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG50.31928480204342274No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGTA40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGTCCAGGT40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTA40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACCGGTCCAGGG40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAATACCTGTCCAGTG40.2554278416347382No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTATCGCGCCTCAGTGTCAGAGCTGGTCTAGCT40.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGGGCCAGTG30.19157088122605362No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGCGTCAGTTCTAAGCCAGGA30.19157088122605362No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGTGCCAGAT30.19157088122605362No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA30.19157088122605362No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG30.19157088122605362No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGAGGTC30.19157088122605362No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC30.19157088122605362No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCG20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCCCAACGTCAGTTACCGTCCAGTA20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGTT20.1277139208173691No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGCCCCAGAG20.1277139208173691No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA20.1277139208173691No Hit
GATACCGCGGATGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC20.1277139208173691Illumina Single End PCR Primer 1 (95% over 21bp)
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG20.1277139208173691No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGCTGGTGCCCAGTA20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG20.1277139208173691No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTTTCAGTCCAGGT20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACAGGTCCAGGG20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTTACAGTCCAGTA20.1277139208173691No Hit
CCTATTTGCTCCCCACACTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT20.1277139208173691No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20.1277139208173691No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGCG20.1277139208173691No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATTTCGGTGGTC20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGTCCAGTG20.1277139208173691No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG20.1277139208173691No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCCGA20.1277139208173691No Hit
TGTGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA20.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA20.1277139208173691No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCTGTGTAGCC20.1277139208173691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)