FastQCFastQC Report
Tue 30 May 2023
EGAF00006171455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006171455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT12935.342465753424655No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG3710.136986301369863No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCGTAGTA184.931506849315069No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC174.657534246575342No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGGCCAGTG143.8356164383561646No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGTCCAGGT71.9178082191780823No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCGGGCCAGTG51.36986301369863No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGGGCCAGTG51.36986301369863No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA51.36986301369863No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACCGGTCCAGGG41.095890410958904No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG41.095890410958904No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGGT30.821917808219178No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG30.821917808219178No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG30.821917808219178No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.547945205479452No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG20.547945205479452No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG20.547945205479452No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC20.547945205479452No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTCAGTGTCAGTATCAGTCCAGGT20.547945205479452No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAACCGGACCAGAC20.547945205479452No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGTT20.547945205479452No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.547945205479452No Hit
CCTGCTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT20.547945205479452No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA20.547945205479452No Hit
CCTGTTTGCTCCCCACGCTTCCGCACCTCAGTGTCAGTAACGGTCCAGGT10.273972602739726No Hit
AATGATACCGCGGCGGATGGCACACAATTACCATAGTGTAGATCTCGGTG10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCCTAGTA10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCTGTCCAGCC10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCCCAGTGTCAGTATCAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGCCCAGGT10.273972602739726No Hit
TACGCTTTCGCCCACTCACCCATCCATCTACTACAGTCTTCCTCCACCTT10.273972602739726No Hit
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTCAGCCCAGAG10.273972602739726No Hit
CCTGTCTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACCGGTCCAGGG10.273972602739726No Hit
GATACTGCGGCTGCTGGTACAGAATTACCTAGGGTAGATCTAGGTGGTCG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACAGGTCCAGGT10.273972602739726No Hit
CCCGCTTAGGCTAGGCCATCAGCAGGACGACAGGGGCCGTAAACCTGTTT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGTGTCAGTATCATTCCAGGT10.273972602739726No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGTAGTCCAGTT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCCCAGTA10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACAGGTCCAGGG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATGGTCCAGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGCGCCAGTATCAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCGCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTGTCAGTCCCGGT10.273972602739726No Hit
AATGCTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG10.273972602739726No Hit
CCTGTTTGCTCCACACGCTTTCGCACCTCAGTGTCAGGATTAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCGCCACACGCTTACGTGCATGAGCGTCAGTACAGGTCCAGGG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACCGGTCCAGGG10.273972602739726No Hit
CATGCTACCGCGGCTCATTCCACACACCTACCATAGTATAGATTACGGAG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTACGCACCCCCGTGTCAGTCTCAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCACGAGCGTCAGTACAGGTCCAGGG10.273972602739726No Hit
CCTGCTTGCTCCCCACACTTTCCTGCATGAGCGTCAGTTTCAGCCCAGCG10.273972602739726No Hit
CCTTTTTTGTCCACCCGCTTACTGTCCCCCCTGTCCTTTTCCATTTCTCC10.273972602739726No Hit
CATGATACCGCTGCTGATGGCACACAATTACCACAGTGTAGAACACTGAG10.273972602739726No Hit
GATACCGCGGCGGCTGGCACACAATTACCATCGTGTAGATCTCGGTGGTC10.273972602739726Illumina RNA PCR Primer (95% over 21bp)
CCTGTTTGCTCCCCTCGCTTTCGTGCCTCAGCGTCAGTATAGGCCTAGTA10.273972602739726No Hit
AGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGTGTCAGTAATAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCGTAGTA10.273972602739726No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAATATTAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGA10.273972602739726No Hit
CATGTTTGATCTGCACGCTCCTGCCTATCAGCGTGAGTATAAATTATGTC10.273972602739726No Hit
CCTGTTTGATCCCCACGCCTTCGTGCCTCAGCGTCAATATATGCGTAGTA10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCACTATATGCGTAGTA10.273972602739726No Hit
CCTTCTCTGGCCGCTCGCTTACCATTATCATAGTGTATTTCGCTACGGTG10.273972602739726No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTCGATCTCGGTGGTC10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAGATGCGTAGTA10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCAGTACAGGTCCAGTG10.273972602739726No Hit
CCTGTTTGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTAACGGGCCAGTG10.273972602739726No Hit
AATGATACCGCGGCTGCTTGCACACAACTACCATAGTGTAGATCTCGGTG10.273972602739726No Hit
AATGATACCGCTGAGGCGACCACACAAATACCAGAGTCACGAACAAATAG10.273972602739726No Hit
AATGATACCGCGGCGGCTGGCACACAATCATCATAGTGTAGATCTTGGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTACCGGGCCAGTG10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCCATATATGTGTAGCC10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCGGTATTAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCACACGCTTTCGCAGCTCAGTGTCCGTATCAGTCCAGGT10.273972602739726No Hit
CATGATACCGCGGCGGCTGGCACACAATTACCACAGTGTATATCTAAGAT10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGGCAGTTTATGCGTAGTA10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGTCCAGTG10.273972602739726No Hit
CCTGTTTACTCCCCTCACTTTCCCACCTCAGCCTCCGTCTCCGTCCCCTT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTCTCAGTCCAGGT10.273972602739726No Hit
CATGATATCGCGGCGCCTGGCACACAATTACCATAGTGTAGATCACGGTG10.273972602739726No Hit
TATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCA10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTAACAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCATGAGCGTCAGTACAGGTCCAGGG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCAGTTCAGGTCCAGTG10.273972602739726No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGCTCTCGGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGCACAGGTCCAGGG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATTAGCGTCAGTACATGTCCAGTG10.273972602739726No Hit
CCTGTTTACTCCCCACTCTTTCGCACCTCAGCCTCAGTATCAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTGGCACCTCAGGGGCAGAACCAGTCCAGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTATCAGTCCAGTT10.273972602739726No Hit
AATGATACCGCTGCGGCTGGCACACACTTACCATAGTGTAGATCTCGGAT10.273972602739726No Hit
CCTGTTTGCTCCTCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCG10.273972602739726No Hit
AATGATACCGCTGAGGCTGCCACACAATTACCATAGTGTATATCAATGAT10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCTGTGTAGCC10.273972602739726No Hit
CCTGTTTACTCCCCACGCTTTCGCACCTCAGTCTCGGTATCAGTCCAGCT10.273972602739726No Hit
CATACCGCGGCGGCTGGCACACAATCACCATAGTGTAGATCTCGGTGGTC10.273972602739726No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCT10.273972602739726No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC10.273972602739726No Hit
CATGATACCTCGGCTGCCTGCACACAATTACCATAGGGTAGAGCTCAGTA10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCCGGTCCAGGG10.273972602739726No Hit
CCTGTTTCCTCCCCACACTTACGCCCCTCAGTGTCCGGCTCAGTCCACTT10.273972602739726No Hit
AATGATACCGCGGCTGCTGGCACACAATTCACATAGTGTAGATTTAGGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTACGCACCTCAGTGTAAGTATCAGTCCAGGT10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTACGAGCCTCAGCGTCAGTTCATGCCCAGTA10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCAGTTCCCGTCCAGTG10.273972602739726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGCGCGTCAGTACCGGTCCAGGG10.273972602739726No Hit
CATACCGCGGCGGCTGGCACACAATTACCATCGTGTAGATCTCGGTGTTC10.273972602739726No Hit
AATGATACCGCGGCGGATGGCACACAACTACCATCGTGTAGATCTCGGTG10.273972602739726No Hit
CCTGTTTACTCCCCACCCTTTCCCGCCTCCTCGTCCGTTTTCCTCCATTT10.273972602739726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)