FastQCFastQC Report
Tue 7 May 2024
EGAF00006171468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006171468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030
Total Bases206 kbp
Sequences flagged as poor quality0
Sequence length200
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA11911.553398058252426No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA757.281553398058252No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA626.019417475728155No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA525.048543689320388No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA434.174757281553398No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA363.4951456310679614No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA292.815533980582524No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA282.7184466019417477No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA242.3300970873786406No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA222.1359223300970873No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA181.7475728155339807No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA171.650485436893204No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA111.0679611650485437No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA111.0679611650485437No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT111.0679611650485437No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT100.9708737864077669No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT100.9708737864077669No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGAGGGAGCA100.9708737864077669No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA90.8737864077669903No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA90.8737864077669903No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT70.6796116504854369No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT70.6796116504854369No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA70.6796116504854369No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA70.6796116504854369No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA70.6796116504854369No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT70.6796116504854369No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA70.6796116504854369No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA70.6796116504854369No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT60.5825242718446602No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT60.5825242718446602No Hit
TACCGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA60.5825242718446602No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT60.5825242718446602No Hit
TACAGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCGCGTA60.5825242718446602No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT60.5825242718446602No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT60.5825242718446602No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA60.5825242718446602No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA60.5825242718446602No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC60.5825242718446602No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATAATTGGGCGTAAAGGGCGCGTA60.5825242718446602No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT50.48543689320388345No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT50.48543689320388345No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA50.48543689320388345No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC40.3883495145631068No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA40.3883495145631068No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA40.3883495145631068No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA40.3883495145631068No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC40.3883495145631068No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT40.3883495145631068No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTAGGTGTAAAGGGAGCGCA30.2912621359223301No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC30.2912621359223301No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT30.2912621359223301No Hit
TACGTATGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA30.2912621359223301No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC30.2912621359223301No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC30.2912621359223301No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC30.2912621359223301No Hit
TACGTAGGTAGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA30.2912621359223301No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA30.2912621359223301No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT30.2912621359223301No Hit
GACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGAGTGTA30.2912621359223301No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC30.2912621359223301No Hit
TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA30.2912621359223301No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA30.2912621359223301No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC20.1941747572815534No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTC20.1941747572815534No Hit
TGCGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA20.1941747572815534No Hit
TACGAAGGGGGCTAGCGTTGTACGGAATCACTGGGCGTAAAGCGCACGTA20.1941747572815534No Hit
TTAGAAACCCGAGTAGTCCGGCTGACTGACTCGTTTAGAGTCGATCTCGT20.1941747572815534No Hit
TACGTAGGGCGCAAGTGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA20.1941747572815534No Hit
AACGTAGGCCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA20.1941747572815534No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGCA20.1941747572815534No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAAATC20.1941747572815534No Hit
TACGGAGGATGCGAGCGTTATTCGGAATCATTGGGTTTAAAGGGTCTGTA20.1941747572815534No Hit
AACGTAGGTCGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA20.1941747572815534No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA20.1941747572815534No Hit
TACGTATGGTGCAGGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA20.1941747572815534No Hit
CATTATTAGAAACCACGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT20.1941747572815534No Hit
TACGTAGGGTGCGAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGCA20.1941747572815534No Hit
TACGTAGGGTGCAAGCGTTAACCGGAATTACTGGGCGTAAAGCGTGCGCA20.1941747572815534No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACCGGGTGTAAAGGGAGCGTA20.1941747572815534No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.1941747572815534No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTCGTTTAGAGTCGAT20.1941747572815534No Hit
AACATAGGGGGCAAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA20.1941747572815534No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA20.1941747572815534No Hit
TACGCAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA20.1941747572815534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)