FastQCFastQC Report
Wed 31 May 2023
EGAF00006172022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42481
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1550336.4939620065441No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA601714.163979190697018No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA535112.596219486358606No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCA25375.972081636496316No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA20504.8256867776182295No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA10512.4740472211106144No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA9812.309267672606577No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCACGCA8481.9961865304489064No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA7181.6901673689414092No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA5191.221722652479932No Hit
TACGCAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA4100.9651373555236459No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCCCGCA2850.6708881617664367No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCCCGCA2800.6591181940161484No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGTGCA2280.5367105294131493No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCGCGTA2250.5296485487629764No Hit
TACGGAGGGTGCAAGCGTTAACCGGAATTACTGGGCGTAAAGCGCACGCA1400.3295590970080742No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1240.29189520020715143No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGCA1070.25187730985617096No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGAGTGCA950.22362938725547893No Hit
TACGGAGGGTGCAAGCGTTAAGCGGAATTACTGGGCGTAAAGCGCACGCA900.21185941950519058No Hit
TACGCAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCGCGTA830.19538146465478684No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA740.1741955227042678No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGCA690.16242555495397942No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA580.13653162590334503No Hit
TACGTAGGTGGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA530.12476165815305665No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCGCGTA500.11769967750288365No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAGTGCA460.10828370330265295No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGCA460.10828370330265295No Hit
TACGAAGGGTGCAAGCGTTAACCGGAATTACTGGGCGTAAAGCGCGCGTA430.10122172265247993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)