FastQCFastQC Report
Tue 30 May 2023
EGAF00006172028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504
Sequences flagged as poor quality0
Sequence length200
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA442.925531914893617No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT412.7260638297872344No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT392.5930851063829787No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT342.2606382978723407No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT281.8617021276595744No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT261.7287234042553192No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT251.6622340425531914No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT251.6622340425531914No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT251.6622340425531914No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT241.5957446808510638No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT241.5957446808510638No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT241.5957446808510638No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT231.5292553191489362No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT231.5292553191489362No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA221.4627659574468086No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT221.4627659574468086No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT221.4627659574468086No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT211.3962765957446808No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT191.2632978723404253No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAT181.196808510638298No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA161.0638297872340425No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA150.9973404255319149No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC130.8643617021276596No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC130.8643617021276596No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC110.7313829787234043No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC110.7313829787234043No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC110.7313829787234043No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC100.6648936170212766No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA100.6648936170212766No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC90.598404255319149No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC90.598404255319149No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC90.598404255319149No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC90.598404255319149No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC80.5319148936170213No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC80.5319148936170213No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC70.4654255319148936No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC70.4654255319148936No Hit
CATATTAGAAACCCGAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC60.39893617021276595No Hit
CATATTAGATACCCTGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC60.39893617021276595No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA60.39893617021276595No Hit
CATATTAGAAACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC60.39893617021276595No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC60.39893617021276595No Hit
CATATTAGAAACCCGGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC60.39893617021276595No Hit
CATATTAGAAACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC60.39893617021276595No Hit
CATATTAGATACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC60.39893617021276595No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC60.39893617021276595No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA60.39893617021276595No Hit
CATATTAGAAACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC60.39893617021276595No Hit
CATATTAGAAACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC50.3324468085106383No Hit
CATATTAGATACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC50.3324468085106383No Hit
CATATTAGATACCCGAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC50.3324468085106383No Hit
CATATTAGAAACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC50.3324468085106383No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAC50.3324468085106383No Hit
CATATTAGATACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC50.3324468085106383No Hit
CATATTAGATACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC50.3324468085106383No Hit
CATATTAGATACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC50.3324468085106383No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA40.26595744680851063No Hit
TTAGAAACCCTGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTCGT40.26595744680851063No Hit
CATATTAGAAACCCTGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC40.26595744680851063No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAA40.26595744680851063No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAA40.26595744680851063No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA40.26595744680851063No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCACCTC30.19946808510638298No Hit
CATATTAGAAACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC30.19946808510638298No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC30.19946808510638298No Hit
TTAGAAACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTCGT30.19946808510638298No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTC30.19946808510638298No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCACCTC30.19946808510638298No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAA30.19946808510638298No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAA30.19946808510638298No Hit
TTAGATACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTCGT30.19946808510638298No Hit
TTAGATACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTCGT20.13297872340425532No Hit
AGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAG20.13297872340425532No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAC20.13297872340425532No Hit
GGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGC20.13297872340425532No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCACCTC20.13297872340425532No Hit
CATATTAGATACCCGGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATC20.13297872340425532No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAA20.13297872340425532No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA20.13297872340425532No Hit
TTAGAAACCCCTGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTCGT20.13297872340425532No Hit
TTCAGCGAAACCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAAC20.13297872340425532No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA20.13297872340425532No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.13297872340425532No Hit
TTAGAAACCCCGGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTCGT20.13297872340425532No Hit
GGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTC20.13297872340425532No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCACGCAT20.13297872340425532No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTTCAAGCCCAAGCAT20.13297872340425532No Hit
TTAGAAACCCTAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCATCTCGT20.13297872340425532No Hit
TACGTAGGTGGCAAGTGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA20.13297872340425532No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA20.13297872340425532No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTTCAAGCTCAAGCAA20.13297872340425532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)