FastQCFastQC Report
Tue 30 May 2023
EGAF00006172029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504
Sequences flagged as poor quality0
Sequence length200
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG16410.904255319148938No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG1348.909574468085106No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC603.9893617021276597No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC463.0585106382978724No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA352.327127659574468No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG241.5957446808510638No Hit
ATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG231.5292553191489362No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA201.3297872340425532No Hit
ANTGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG201.3297872340425532No Hit
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG201.3297872340425532No Hit
ATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG191.2632978723404253No Hit
AATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG191.2632978723404253No Hit
AATGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG110.7313829787234043No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC100.6648936170212766Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG100.6648936170212766No Hit
TACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC100.6648936170212766Illumina Single End PCR Primer 1 (100% over 21bp)
AATGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG80.5319148936170213No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA80.5319148936170213No Hit
GNTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC70.4654255319148936No Hit
ANGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG70.4654255319148936No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAATACCATAGTA60.39893617021276595No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGAG60.39893617021276595No Hit
GNTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC50.3324468085106383No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA40.26595744680851063No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTAGGTG40.26595744680851063No Hit
ATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG40.26595744680851063No Hit
GGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC40.26595744680851063No Hit
AATGATACCGCGGCGGCGGGCACACAATTACCATAGTGTAGATCTCGGTG30.19946808510638298No Hit
AATGATACCGCGGCGGCCGGCACACAATTACCATAGTGTAGATCTCGGTG30.19946808510638298No Hit
TNCCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC30.19946808510638298Illumina Single End PCR Primer 1 (95% over 23bp)
CNTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAATACCATAGTA30.19946808510638298No Hit
CNTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA30.19946808510638298No Hit
ANTGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG30.19946808510638298No Hit
ANTGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG30.19946808510638298No Hit
ANGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.13297872340425532No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGAC20.13297872340425532No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGCC20.13297872340425532No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG20.13297872340425532No Hit
AATGATACCGCGGCAGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.13297872340425532No Hit
GTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC20.13297872340425532No Hit
ATGATACCGCGGCGGCTGGCACACCATTACCATAGTGTAGATCTCGGTGG20.13297872340425532No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGGG20.13297872340425532No Hit
AGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTC20.13297872340425532No Hit
CTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATT20.13297872340425532No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA20.13297872340425532No Hit
TNCCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC20.13297872340425532Illumina Single End PCR Primer 1 (95% over 23bp)
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA20.13297872340425532No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG20.13297872340425532No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGAG20.13297872340425532No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG20.13297872340425532No Hit
ATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.13297872340425532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)