FastQCFastQC Report
Sun 21 May 2023
EGAF00006172030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488
Sequences flagged as poor quality0
Sequence length200
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGAT763.054662379421222No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGAT702.8135048231511255No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGAT692.7733118971061095No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTTAATCCACAGCGAT692.7733118971061095No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGAT662.652733118971061No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTTAATCCACAGCGAT642.572347266881029No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGAT642.572347266881029No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTTAATCCACAGCGAT632.532154340836013No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTTAATCCACAGCGAT491.969453376205788No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGAT471.8890675241157555No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGAT471.8890675241157555No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGAT461.8488745980707395No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTTAATCCACAGCGAT411.647909967845659No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGAT391.567524115755627No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGAT391.567524115755627No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGAT331.3263665594855305No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTTAATCCACAGCGAT311.2459807073954985No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGAT291.1655948553054662No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC291.1655948553054662No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA271.0852090032154342No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC261.045016077170418No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC261.045016077170418No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC251.004823151125402No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA251.004823151125402No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC240.964630225080386No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC210.8440514469453377No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC210.8440514469453377No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA170.6832797427652734No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC170.6832797427652734No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA170.6832797427652734No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA160.6430868167202572No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC150.6028938906752411No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC140.5627009646302251No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC130.522508038585209No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA130.522508038585209No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC120.482315112540193No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC120.482315112540193No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC110.44212218649517687No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC100.40192926045016075No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC90.3617363344051447No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC90.3617363344051447No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA90.3617363344051447No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTC90.3617363344051447No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGAA70.28135048231511256No Hit
TTAGAAACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT70.28135048231511256No Hit
TTAGAAACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT70.28135048231511256No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA70.28135048231511256No Hit
TTAGATACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT60.2411575562700965No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGAA60.2411575562700965No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA60.2411575562700965No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGAA60.2411575562700965No Hit
TTAGATACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT60.2411575562700965No Hit
TTAGAAACCCGAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT60.2411575562700965No Hit
TTAGAAACCCTGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT50.20096463022508038No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGAA50.20096463022508038No Hit
TTAGATACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT50.20096463022508038No Hit
TTAGATACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT50.20096463022508038No Hit
CATATTAGAAACCCTGGTAGTCCGGCTGACTGACTTAATCCACAGCGATC50.20096463022508038No Hit
TTAGAAACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT50.20096463022508038No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA50.20096463022508038No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGAA50.20096463022508038No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGACCTC40.1607717041800643No Hit
CATATTAGAAACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGATC40.1607717041800643No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGAA40.1607717041800643No Hit
CATTATTAGAACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGATC40.1607717041800643No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA40.1607717041800643No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGAA40.1607717041800643No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTTAATCCACAGCGAC40.1607717041800643No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGAA40.1607717041800643No Hit
TTAGAAACCCGGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT30.12057877813504825No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGAC30.12057877813504825No Hit
TTAGATACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT30.12057877813504825No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGAC30.12057877813504825No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA30.12057877813504825No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA30.12057877813504825No Hit
TTAGAAACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGATCTCGT30.12057877813504825No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGAA30.12057877813504825No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTTAATCCACAGCGAA30.12057877813504825No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTTAATCCACAGCGAA30.12057877813504825No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTTAATCCACAGCGAA30.12057877813504825No Hit
ACAATTAGATACCCTAGTAGTCCGGCTGACTGACTTAATCCACAGCGATC30.12057877813504825No Hit
CATATTAGAAACCCGTGTAGTCCGGCTGACTGACTTAATCCACAGCGATC30.12057877813504825No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGAA30.12057877813504825No Hit
CATTTAGAAACCCGGGTAGTCCGGCTGACTGACTTAATCCACAGCGATCT30.12057877813504825No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTTAATCCACAGCGAA30.12057877813504825No Hit
ACAATTAGAAACCCCGGTAGTCCGGCTGACTGACTTAATCCACAGCGATC30.12057877813504825No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTTAATCCACAGCGAA30.12057877813504825No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA30.12057877813504825No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTTAATCCACAGCGACCTC30.12057877813504825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)