FastQCFastQC Report
Tue 30 May 2023
EGAF00006172031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488
Sequences flagged as poor quality0
Sequence length200
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG34613.906752411575562No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG34213.745980707395496No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC1084.340836012861737No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC1064.260450160771704No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG481.929260450160772No Hit
ANTGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG391.567524115755627No Hit
TACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC301.2057877813504823Illumina Single End PCR Primer 1 (100% over 21bp)
AATGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG261.045016077170418No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC240.964630225080386Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA220.8842443729903537No Hit
AATGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG190.7636655948553055No Hit
CCTGTTTGCTCCCTACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG180.7234726688102894No Hit
AATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG150.6028938906752411No Hit
GNTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC150.6028938906752411No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG130.522508038585209No Hit
GNTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC120.482315112540193No Hit
TNCCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC120.482315112540193Illumina Single End PCR Primer 1 (95% over 23bp)
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG110.44212218649517687No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGAG100.40192926045016075No Hit
ATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG90.3617363344051447No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA80.3215434083601286No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATTTCGGTG80.3215434083601286No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA80.3215434083601286No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTAGGTG70.28135048231511256No Hit
GGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC70.28135048231511256No Hit
ATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG60.2411575562700965No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTAGGTG60.2411575562700965No Hit
TNCCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC60.2411575562700965Illumina Single End PCR Primer 1 (95% over 23bp)
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTTGGTG50.20096463022508038No Hit
GGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC50.20096463022508038No Hit
GTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC50.20096463022508038No Hit
AATGATACCGCGGCGGCGGGCACACAATTACCATAGTGTAGATCTCGGTG40.1607717041800643No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG40.1607717041800643No Hit
AATGATACCGCGGCGGCCGGCACACAATTACCATAGTGTAGATCTCGGTG40.1607717041800643No Hit
ANTGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG40.1607717041800643No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATTTCGGTG40.1607717041800643No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGAG40.1607717041800643No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG40.1607717041800643No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA40.1607717041800643No Hit
ANTGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG40.1607717041800643No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATTTCGGTG40.1607717041800643No Hit
AATGATACCGCGGCGGCTGGCACACCATTACCATAGTGTAGATCTCGGTG30.12057877813504825No Hit
AATGATACCGCGGCTGCTGGCACACAACTACCATAGTGTAGATCTCGGTG30.12057877813504825No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGAG30.12057877813504825No Hit
GTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC30.12057877813504825No Hit
CNTGTTTGCTCCCTACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG30.12057877813504825No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTTGGTG30.12057877813504825No Hit
CNTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA30.12057877813504825No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG30.12057877813504825No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA30.12057877813504825No Hit
ANGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG30.12057877813504825No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA30.12057877813504825No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA30.12057877813504825No Hit
TGTGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG30.12057877813504825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)