FastQCFastQC Report
Tue 30 May 2023
EGAF00006172033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688
Sequences flagged as poor quality0
Sequence length200
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG23814.099526066350712No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG23613.981042654028435No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC824.857819905213271No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC663.909952606635071No Hit
ANTGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG392.31042654028436No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG352.0734597156398102No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA271.5995260663507107No Hit
GTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC181.066350710900474No Hit
AATGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG181.066350710900474No Hit
AATGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG140.8293838862559242No Hit
ATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG120.7109004739336493No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGAG110.6516587677725119No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC100.5924170616113744Illumina Single End PCR Primer 1 (100% over 21bp)
GNTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC100.5924170616113744No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA90.533175355450237No Hit
GNTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC90.533175355450237No Hit
GTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC90.533175355450237No Hit
GGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC80.47393364928909953No Hit
AATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG80.47393364928909953No Hit
ATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG70.4146919431279621No Hit
CNTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA60.35545023696682465No Hit
TACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC60.35545023696682465Illumina Single End PCR Primer 1 (100% over 21bp)
CNTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAATACCATAGTA60.35545023696682465No Hit
AATGATACCGCGGCGGCGGGCACACAATTACCATAGTGTAGATCTCGGTG50.2962085308056872No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTAATGGCCCAGAG50.2962085308056872No Hit
ANGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG50.2962085308056872No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA40.23696682464454977No Hit
GGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC40.23696682464454977No Hit
AATGATACCGCGGCTGCTGGCACACCATTACCATAGTGTAGATCTCGGTG40.23696682464454977No Hit
AATGATACCGCGGCGGCTGGCACACAACTACCATAGTGTAGATCTCGGTG30.17772511848341233No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG30.17772511848341233No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGAG30.17772511848341233No Hit
AATGATACCGCGGCGGCCGGCACACAATTACCATAGTGTAGATCTCGGTG30.17772511848341233No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTAGGTG30.17772511848341233No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG30.17772511848341233No Hit
TNCCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC30.17772511848341233Illumina Single End PCR Primer 1 (95% over 23bp)
CATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG30.17772511848341233No Hit
AATGATACCGCGGCTGCTGGCACACAACTACCATAGTGTAGATCTCGGTG30.17772511848341233No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAATACCATAGTA30.17772511848341233No Hit
AATGATACCGCGGCTGCTGGCACACAATTACTATAGTGTAGATCTTGGTG30.17772511848341233No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATTTCGGTG20.11848341232227488No Hit
ANGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.11848341232227488No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTTGGTG20.11848341232227488No Hit
ATGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.11848341232227488No Hit
GATACTGCGGCGGCTGGCACACAATTACCATAGTGTAGTTCTCGGTGGTC20.11848341232227488No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG20.11848341232227488No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAATACCACAGTA20.11848341232227488No Hit
GNTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGGC20.11848341232227488No Hit
GAAGTGGACTGCTGGCGGAAAATGAGAAACTTCGACCTATCCTTGCGCAG20.11848341232227488No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCTGTG20.11848341232227488No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA20.11848341232227488No Hit
AATGCTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.11848341232227488No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG20.11848341232227488No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGCG20.11848341232227488No Hit
AATGATACCGCGGCGGCTGGAACACAATTACCATAGTGTAGATCTCGGTG20.11848341232227488No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG20.11848341232227488No Hit
ATTTTAGTAAGCTCTTTTTGATTCTCAAATCCGGCGTCAACCATACCAGC20.11848341232227488No Hit
ATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.11848341232227488No Hit
CNTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG20.11848341232227488No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGAG20.11848341232227488No Hit
ANTGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.11848341232227488No Hit
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.11848341232227488No Hit
ACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGCC20.11848341232227488Illumina Single End PCR Primer 1 (100% over 22bp)
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTTGTC20.11848341232227488No Hit
TGTGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.11848341232227488No Hit
AATGATACCGCGGCTGCTGGCACACAATTACATAGTGTAGATCTCGGTGG20.11848341232227488No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG20.11848341232227488No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGGGTAGATCTCGGTG20.11848341232227488No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20.11848341232227488No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATCGTGTAGATCTCGGTG20.11848341232227488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)