FastQCFastQC Report
Tue 30 May 2023
EGAF00006172036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7070
Sequences flagged as poor quality0
Sequence length200
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA122017.256011315417254No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA3014.257425742574258No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2483.507779349363508No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA2433.437057991513437No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA2163.0551626591230554No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1452.050919377652051No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA1351.9094766619519095No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTATGGACCGAACCAT1281.8104667609618104No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1241.753889674681754No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1171.654879773691655No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTATGGACCGAACCAT1141.6124469589816126No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTATGGACCGAACCAT991.4002828854314004No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTATGGACCGAACCAT981.3861386138613863No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTATGGACCGAACCAT881.2446958981612446No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA861.2164073550212162No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTATGGACCGAACCAT811.1456859971711457No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA791.1173974540311173No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTATGGACCGAACCAT721.0183875530410185No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTATGGACCGAACCAT721.0183875530410185No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTATGGACCGAACCAT690.9759547383309759No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTATGGACCGAACCAT630.891089108910891No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTATGGACCGAACCAT610.8628005657708628No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTATGGACCGAACCAT610.8628005657708628No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTATGGACCGAACCAT580.8203677510608205No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA580.8203677510608205No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTATGGACCGAACCAT550.7779349363507779No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTATGGACCGAACCAT540.7637906647807639No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTATGGACCGAACCAT540.7637906647807639No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA530.7496463932107497No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTATGGACCGAACCAT470.6647807637906648No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC440.6223479490806223No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC380.5374823196605375No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA360.5091937765205092No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC360.5091937765205092No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA340.48090523338048086No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA330.4667609618104668No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA330.4667609618104668No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC320.4526166902404526No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC320.4526166902404526No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC290.41018387553041025No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTATGGACCGAACCAT270.3818953323903819No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA260.3677510608203678No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA260.3677510608203678No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA250.3536067892503536No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC240.33946251768033947No Hit
TACGTAGGGGGCAAGTGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA240.33946251768033947No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC240.33946251768033947No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC230.32531824611032534No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA230.32531824611032534No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA220.31117397454031115No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA220.31117397454031115No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC210.297029702970297No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA210.297029702970297No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA200.2828854314002829No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA190.26874115983026875No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA190.26874115983026875No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA190.26874115983026875No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC190.26874115983026875No Hit
AACGTAGGTCACGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA180.2545968882602546No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC180.2545968882602546No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA180.2545968882602546No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC170.24045261669024043No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC170.24045261669024043No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA170.24045261669024043No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA160.2263083451202263No Hit
CATATTAGAAACCCCTGTAGTCCGGCTGACTGACTATGGACCGAACCATC150.21216407355021216No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC150.21216407355021216No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA140.19801980198019803No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC140.19801980198019803No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA140.19801980198019803No Hit
GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTA140.19801980198019803No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA130.1838755304101839No Hit
CATATTAGAAACCCCGGTAGTCCGGCTGACTGACTATGGACCGAACCATC130.1838755304101839No Hit
TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA120.16973125884016974No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTATGGACCGAACCAA110.15558698727015557No Hit
TACGTATGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA110.15558698727015557No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTATGGACCGAACCAA100.14144271570014144No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA100.14144271570014144No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA100.14144271570014144No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC100.14144271570014144No Hit
CATATTAGAAACCCCAGTAGTCCGGCTGACTGACTATGGACCGAACCATC100.14144271570014144No Hit
TACGTAGGGGGCAGGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA90.1272984441301273No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTATGGACCGAACCAA90.1272984441301273No Hit
TACGTAGGTAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA90.1272984441301273No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA90.1272984441301273No Hit
CATATTAGAAACCCGAGTAGTCCGGCTGACTGACTATGGACCGAACCATC80.11315417256011315No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTATGGACCGAACCAA80.11315417256011315No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTATGGACCGAACCATCTC80.11315417256011315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)