FastQCFastQC Report
Sun 21 May 2023
EGAF00006172038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533
Sequences flagged as poor quality0
Sequence length200
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA2244.941539819104346No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1954.301786896095301No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1503.3090668431502315No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1002.2060445621001543No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA972.13986322523715No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA851.8751378777851313No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA791.742775204059122No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA551.2133245091550848No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT541.1912640635340834No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT481.0589013898080741No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT471.0368409441870725No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT440.9706596073240679No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA440.9706596073240679No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT430.9485991617030664No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA390.8603573792190603No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA380.8382969335980588No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT370.8162364879770571No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT360.7941760423560555No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT360.7941760423560555No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT350.772115596735054No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTA330.727994705493051No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT320.7059342598720494No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC320.7059342598720494No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA320.7059342598720494No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC310.6838738142510479No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT300.6618133686300464No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA290.6397529230090447No Hit
CACCGGCAGCTCTAGTGGTAGCAGTTTTTATTGGGCCTAAAGCGTCCGTA290.6397529230090447No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA290.6397529230090447No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA290.6397529230090447No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT270.5956320317670417No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA270.5956320317670417No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT270.5956320317670417No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT270.5956320317670417No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC260.5735715861460402No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC260.5735715861460402No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA260.5735715861460402No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT250.5515111405250386No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT250.5515111405250386No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC250.5515111405250386No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC230.5073902492830356No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA210.4632693580410324No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC210.4632693580410324No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA210.4632693580410324No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA200.4412089124200309No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC200.4412089124200309No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA190.4191484667990294No Hit
CATATTAGAAACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGATC190.4191484667990294No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC190.4191484667990294No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA190.4191484667990294No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA190.4191484667990294No Hit
TACGTATGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGTA180.3970880211780278No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT180.3970880211780278No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA170.37502757555702626No Hit
TACGGAGGATGCGAGCGTTATCCGGAATTATTGGGTTTAAAGGGTGCGTA170.37502757555702626No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT170.37502757555702626No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC160.3529671299360247No Hit
TTAGAAACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT160.3529671299360247No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC160.3529671299360247No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGTGTA160.3529671299360247No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA160.3529671299360247No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC140.3088462386940216No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA140.3088462386940216No Hit
TCATATTAGATACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT140.3088462386940216No Hit
TTAGAAACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT140.3088462386940216No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA140.3088462386940216No Hit
TCATATTAGAAACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT130.2867857930730201No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC130.2867857930730201No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA130.2867857930730201No Hit
CACGTAGGGGGCGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA130.2867857930730201No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC130.2867857930730201No Hit
TTAGAAACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT130.2867857930730201No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC130.2867857930730201No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGTGCGTA130.2867857930730201No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA120.26472534745201853No Hit
CATATTAGAAACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGATC120.26472534745201853No Hit
TCATATTAGATACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT120.26472534745201853No Hit
TCATATTAGAAACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT110.24266490183101697No Hit
TTAGAAACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT110.24266490183101697No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC110.24266490183101697No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC110.24266490183101697No Hit
TTAGAAACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT110.24266490183101697No Hit
TTAGATACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT110.24266490183101697No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGTGTA110.24266490183101697No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA100.22060445621001545No Hit
TTAGAAACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT100.22060445621001545No Hit
CATATTAGATACCCCTGTAGTCCGGCTGACTGACTAAGATGGATCAGATC100.22060445621001545No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTA100.22060445621001545No Hit
TCATATTAGAAACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT100.22060445621001545No Hit
TCATATTAGAAACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT100.22060445621001545No Hit
TTAGATACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT90.1985440105890139No Hit
TTAGATACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT90.1985440105890139No Hit
CATATTAGAAACCCGGGTAGTCCGGCTGACTGACTAAGATGGATCAGATC80.17648356496801235No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGCGTA80.17648356496801235No Hit
TTAGATACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT80.17648356496801235No Hit
CGGCAGCGGTACCACCACGCATCTGCATTAGAAACCCTTGTAGTCCGGCT80.17648356496801235No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTC80.17648356496801235No Hit
CATATTAGAAACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGATC80.17648356496801235No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA80.17648356496801235No Hit
TCATATTAGAAACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT80.17648356496801235No Hit
TTAGAAACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT80.17648356496801235No Hit
TCATATTAGAAACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT80.17648356496801235No Hit
TCATATTAGATACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT80.17648356496801235No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA70.1544231193470108No Hit
CGGCAGCGGTACCACCACGCATCTGCATTAGAAACCCCGGTAGTCCGGCT70.1544231193470108No Hit
TCATATTAGAAACCCGGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT70.1544231193470108No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA70.1544231193470108No Hit
TCATATTAGAAACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT70.1544231193470108No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA70.1544231193470108No Hit
CATATTAGATACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGATC70.1544231193470108No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA70.1544231193470108No Hit
TTAGATACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT70.1544231193470108No Hit
TCATATTAGATACCCTTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT60.13236267372600927No Hit
CATATTAGAAACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGATC60.13236267372600927No Hit
TACGGAGGGTGCAAGTGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA60.13236267372600927No Hit
TCATATTAGATACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGAT60.13236267372600927No Hit
TTAGATACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT60.13236267372600927No Hit
CGGCAGCGGTACCACCACGCATCTGCATTAGAAACCCGAGTAGTCCGGCT60.13236267372600927No Hit
CGGCAGCGGTACCACCACGCATCTGCATTAGAAACCCTGGTAGTCCGGCT60.13236267372600927No Hit
CATATTAGATACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGATC60.13236267372600927No Hit
CATATTAGATACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGATC60.13236267372600927No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA60.13236267372600927No Hit
CATATTAGATACCCTGGTAGTCCGGCTGACTGACTAAGATGGATCAGATC60.13236267372600927No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATAATTGGGCGTAAAGGGCGCGTA60.13236267372600927No Hit
CATATTAGAAACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGATC60.13236267372600927No Hit
TTAGAAACCCGGGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT50.11030222810500773No Hit
TACGGAGGATCCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA50.11030222810500773No Hit
CATATTAGATACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGATC50.11030222810500773No Hit
TCATATTAGATACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT50.11030222810500773No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTAAGATGGATCAGACCTC50.11030222810500773No Hit
TCATATTAGAAACCCGTGTAGTCCGGCTGACTGACTAAGATGGATCAGAT50.11030222810500773No Hit
TACGGAGGATGCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA50.11030222810500773No Hit
TTAGAAACCCGAGTAGTCCGGCTGACTGACTAAGATGGATCAGATCTCGT50.11030222810500773No Hit
TCATATTAGATACCCCGGTAGTCCGGCTGACTGACTAAGATGGATCAGAT50.11030222810500773No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCACGCA50.11030222810500773No Hit
CGGCAGCGGTACCACCACGCATCTGCATTAGAAACCCCAGTAGTCCGGCT50.11030222810500773No Hit
CATATTAGATACCCTAGTAGTCCGGCTGACTGACTAAGATGGATCAGATC50.11030222810500773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)