FastQCFastQC Report
Sun 21 May 2023
EGAF00006172040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676
Sequences flagged as poor quality0
Sequence length200
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1094.073243647234678No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAAT752.8026905829596416No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA622.3168908819133036No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAAT582.167414050822123No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA572.130044843049327No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAAT562.092675635276532No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAAT531.9805680119581466No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC531.9805680119581466No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC451.6816143497757847No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTAGCCGGCACATAAT451.6816143497757847No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTAGCCGGCACATAAT421.5695067264573992No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA381.4200298953662183No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTAGCCGGCACATAAT371.3826606875934229No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA361.345291479820628No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA341.2705530642750373No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC341.2705530642750373No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTAGCCGGCACATAAT341.2705530642750373No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC311.1584454409566518No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAAT301.1210762331838564No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC271.0089686098654709No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC260.9715994020926756No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAAT260.9715994020926756No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC250.9342301943198804No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTAGCCGGCACATAAT250.9342301943198804No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA250.9342301943198804No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTAGCCGGCACATAAT230.85949177877429No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTAGCCGGCACATAAT230.85949177877429No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC220.8221225710014948No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC220.8221225710014948No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA210.7847533632286996No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC200.7473841554559043No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTAGCCGGCACATAAT200.7473841554559043No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA190.7100149476831091No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA180.672645739910314No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTAGCCGGCACATAAT180.672645739910314No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC180.672645739910314No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATAATTGGGCGTAAAGGGCGCGTA180.672645739910314No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC160.5979073243647235No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA140.523168908819133No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTAGCCGGCACATAAT140.523168908819133No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC130.4857997010463378No Hit
TACGGAGGGTGCGAGCGTTACCCGGATTTACTGGGTGTAAAGGGCGTGTA130.4857997010463378No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTAGCCGGCACATAAT130.4857997010463378No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA130.4857997010463378No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC120.4484304932735426No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA110.4110612855007474No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC110.4110612855007474No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTAGCCGGCACATAAT100.37369207772795215No Hit
CATATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAATC100.37369207772795215No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA100.37369207772795215No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC90.336322869955157No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA90.336322869955157No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA80.29895366218236175No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA80.29895366218236175No Hit
CATATTAGAAACCCCTGTAGTCCGGCTGACTGACTAGCCGGCACATAATC70.2615844544095665No Hit
CATATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAATC70.2615844544095665No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAACCTC70.2615844544095665No Hit
TTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGT70.2615844544095665No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAAA70.2615844544095665No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC70.2615844544095665No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA70.2615844544095665No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA60.2242152466367713No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA60.2242152466367713No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTC60.2242152466367713No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA60.2242152466367713No Hit
CATATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAATC60.2242152466367713No Hit
TTAGAAACCCGGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGT50.18684603886397608No Hit
TACGTATGGAGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA50.18684603886397608No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAAA50.18684603886397608No Hit
TTAGAAACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGT50.18684603886397608No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGAGGGAGCA50.18684603886397608No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA50.18684603886397608No Hit
CATATTAGAAACCCGGGTAGTCCGGCTGACTGACTAGCCGGCACATAATC50.18684603886397608No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAAA50.18684603886397608No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA40.14947683109118087No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA40.14947683109118087No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA40.14947683109118087No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTAGCCGGCACATAAA40.14947683109118087No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAAA40.14947683109118087No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA40.14947683109118087No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA40.14947683109118087No Hit
ACAATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAATC40.14947683109118087No Hit
CATATTAGATACCCCTGTAGTCCGGCTGACTGACTAGCCGGCACATAATC40.14947683109118087No Hit
CATATTAGAAACCCTAGTAGTCCGGCTGACTGACTAGCCGGCACATAATC40.14947683109118087No Hit
CATTAGAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCG30.11210762331838565No Hit
CACAATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAAT30.11210762331838565No Hit
TTAGATACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGT30.11210762331838565No Hit
TACATAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTGCGTA30.11210762331838565No Hit
CATATTAGATACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAATC30.11210762331838565No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTAGCCGGCACATAAA30.11210762331838565No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGCCGGCACATAAA30.11210762331838565No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATATC30.11210762331838565No Hit
TTAGAAACCCCTGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGT30.11210762331838565No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTAGCCGGCACATAAA30.11210762331838565No Hit
TTAGAAACCCTAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGT30.11210762331838565No Hit
TTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGT30.11210762331838565No Hit
TCGCCGATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATA30.11210762331838565No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATAAACTC30.11210762331838565No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTAGCCGGCACATAAA30.11210762331838565No Hit
TAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAATCTCGTA30.11210762331838565No Hit
TACGTAGGTGGCGAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTCCGCA30.11210762331838565No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGCCGGCACATATCTCG30.11210762331838565No Hit
TCGCCGATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATA30.11210762331838565No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGCCGGCACATAAC30.11210762331838565No Hit
CATATTAGAAACCCTGGTAGTCCGGCTGACTGACTAGCCGGCACATAATC30.11210762331838565No Hit
CATATTAGATACCCTGGTAGTCCGGCTGACTGACTAGCCGGCACATAATC30.11210762331838565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)