FastQCFastQC Report
Tue 30 May 2023
EGAF00006172042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3915
Sequences flagged as poor quality0
Sequence length200
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA2586.590038314176246No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1584.035759897828863No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA1273.2439335887611747No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1233.1417624521072796No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1132.8863346104725416No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA1072.7330779054916987No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA1002.554278416347382No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA972.4776500638569603No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA601.532567049808429No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTACGATGCGACCAAT591.5070242656449553No Hit
TACGTAGGGAGCGAGCGTTATCCGGAATTACTGGGCGTAAAGGGCGCGTA521.3282247765006385No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA471.2005108556832695No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTACGATGCGACCAAT431.0983397190293742No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTACGATGCGACCAAT421.0727969348659003No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTACGATGCGACCAAT421.0727969348659003No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA411.0472541507024267No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTACGATGCGACCAAT390.996168582375479No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTACGATGCGACCAAT380.9706257982120051No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTACGATGCGACCAAT370.9450830140485313No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTACGATGCGACCAAT370.9450830140485313No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTACGATGCGACCAAT350.8939974457215836No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA350.8939974457215836No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA350.8939974457215836No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTACGATGCGACCAAT340.8684546615581098No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTACGATGCGACCAAT330.8429118773946359No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTACGATGCGACCAAT330.8429118773946359No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTACGATGCGACCAAT320.8173690932311622No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA300.7662835249042145No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTACGATGCGACCAAT300.7662835249042145No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTACGATGCGACCAAT300.7662835249042145No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA290.7407407407407408No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTACGATGCGACCAAT290.7407407407407408No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC290.7407407407407408No Hit
TACGGGGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA280.7151979565772669No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC270.6896551724137931No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC270.6896551724137931No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC260.6641123882503193No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTACGATGCGACCAAT250.6385696040868455No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA240.6130268199233717No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC220.5619412515964239No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC220.5619412515964239No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA210.5363984674329502No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC200.5108556832694764No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA200.5108556832694764No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC190.48531289910600256No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA190.48531289910600256No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTACGATGCGACCAAT190.48531289910600256No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA190.48531289910600256No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC180.45977011494252873No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA170.4342273307790549No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC150.38314176245210724No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC140.35759897828863346No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC130.33205619412515963No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA130.33205619412515963No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA120.30651340996168586No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC120.30651340996168586No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA120.30651340996168586No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC120.30651340996168586No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGAGTA120.30651340996168586No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC120.30651340996168586No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA120.30651340996168586No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA120.30651340996168586No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC110.28097062579821197No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA110.28097062579821197No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA110.28097062579821197No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA100.2554278416347382No Hit
TACGTATGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA100.2554278416347382No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA100.2554278416347382No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA90.22988505747126436No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA90.22988505747126436No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA90.22988505747126436No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGTGTA90.22988505747126436No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGCA80.20434227330779056No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA80.20434227330779056No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA80.20434227330779056No Hit
TACGGAGGATGCGAGCGTTATCCGGAATTATTGGGTTTAAAGGGTCCGCA80.20434227330779056No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC70.17879948914431673No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTACGATGCGACCAATCTC70.17879948914431673No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA70.17879948914431673No Hit
TACGGAGGATCCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA70.17879948914431673No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGTGTA70.17879948914431673No Hit
TACGTATGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA60.15325670498084293No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGTGTA60.15325670498084293No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAATGCGCGCGTA50.1277139208173691No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA50.1277139208173691No Hit
CATATTAGATACCCCAGTAGTCCGGCTGACTGACTACGATGCGACCAATC50.1277139208173691No Hit
CATATTAGAAACCCCTGTAGTCCGGCTGACTGACTACGATGCGACCAATC50.1277139208173691No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTACGATGCGACCAAA50.1277139208173691No Hit
CATATTAGATACCCTAGTAGTCCGGCTGACTGACTACGATGCGACCAATC40.10217113665389528No Hit
CATATTAGATACCCCGGTAGTCCGGCTGACTGACTACGATGCGACCAATC40.10217113665389528No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA40.10217113665389528No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA40.10217113665389528No Hit
CACCGGCAGCTCTAGTGGTAGCAGTTTTTATTGGGCCTAAAGCGTCCGTA40.10217113665389528No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA40.10217113665389528No Hit
CATATTAGATACCCGTGTAGTCCGGCTGACTGACTACGATGCGACCAATC40.10217113665389528No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA40.10217113665389528No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA40.10217113665389528No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA40.10217113665389528No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA40.10217113665389528No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGTA40.10217113665389528No Hit
CATATTAGATACCCTGGTAGTCCGGCTGACTGACTACGATGCGACCAATC40.10217113665389528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)