FastQCFastQC Report
Tue 30 May 2023
EGAF00006172043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3915
Sequences flagged as poor quality0
Sequence length200
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG2396.104725415070242No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG2015.134099616858237No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1864.75095785440613No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1313.34610472541507No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC1273.2439335887611747No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC1122.8607918263090677No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA761.9412515964240102No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA751.9157088122605364No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG691.7624521072796935No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG671.711366538952746No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA571.4559386973180077No Hit
CNTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG501.277139208173691No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA461.1749680715197957No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA411.0472541507024267No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG370.9450830140485313No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG360.9195402298850575No Hit
ANTGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG350.8939974457215836No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCTATGAGCGTCAGTTCATGGCCAGC350.8939974457215836No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA350.8939974457215836No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG290.7407407407407408No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA270.6896551724137931No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA260.6641123882503193No Hit
GNTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC240.6130268199233717No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG240.6130268199233717No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA230.5874840357598978No Hit
CCTGTTCGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTTATAGTCCAGCA220.5619412515964239No Hit
CCTGTTCGCTCCCCCGGCTCTCGCGCCTCAGCGTCGGTGCCGGCCCAGAG210.5363984674329502No Hit
CCTGTTCTATCCCCACGCTTTCGTGCCTCAGCGTCAGTTACGGATTCGCC210.5363984674329502No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA210.5363984674329502No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA190.48531289910600256No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA170.4342273307790549No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA170.4342273307790549No Hit
ATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG160.4086845466155811No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA160.4086845466155811No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA160.4086845466155811No Hit
GNTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC160.4086845466155811No Hit
ATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG150.38314176245210724No Hit
AATGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG150.38314176245210724No Hit
CNTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG140.35759897828863346No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA130.33205619412515963No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA120.30651340996168586No Hit
GTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC120.30651340996168586No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA120.30651340996168586No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA120.30651340996168586No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA110.28097062579821197No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA110.28097062579821197No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA100.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA100.2554278416347382No Hit
CNTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG100.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTCTTCGTCCAGGG100.2554278416347382No Hit
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG100.2554278416347382No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA90.22988505747126436No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA90.22988505747126436No Hit
AATGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG90.22988505747126436No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA90.22988505747126436No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATGATGACCAGAA90.22988505747126436No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA90.22988505747126436No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA90.22988505747126436No Hit
GTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC90.22988505747126436No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCCAGTCCAGA90.22988505747126436No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA90.22988505747126436No Hit
CNCGTTTGCTACCCTAGCTTTCGCGCTATGAGCGTCAGTTCATGGCCAGC90.22988505747126436No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA80.20434227330779056No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA80.20434227330779056No Hit
GGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC80.20434227330779056No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA80.20434227330779056No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA70.17879948914431673No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATCGTCCAGTA70.17879948914431673No Hit
CNTGTTCGCTCCCCCGGCTCTCGCGCCTCAGCGTCGGTGCCGGCCCAGAG70.17879948914431673No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA70.17879948914431673No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA70.17879948914431673No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG70.17879948914431673No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG70.17879948914431673No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA70.17879948914431673No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA60.15325670498084293No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA60.15325670498084293No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA60.15325670498084293No Hit
CCTGTTTGCTCCCCACGCTTTCGTCCCTGAGCGTCAGAATAGTCTTCGTC60.15325670498084293No Hit
CCTGTTTGCTCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAA60.15325670498084293No Hit
CNTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG60.15325670498084293No Hit
CNTGTTCTATCCCCACGCTTTCGTGCCTCAGCGTCAGTTACGGATTCGCC60.15325670498084293No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA60.15325670498084293No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA60.15325670498084293No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG60.15325670498084293No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGTCCAGGT50.1277139208173691No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT50.1277139208173691No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTAATGGCCCAGAG50.1277139208173691No Hit
AATGATACCGCGGCGGCCGGCACACAATTACCATAGTGTAGATCTCGGTG50.1277139208173691No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA50.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA50.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA50.1277139208173691No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTCGTCCAGGG50.1277139208173691No Hit
ANGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG50.1277139208173691No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA50.1277139208173691No Hit
ANGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG40.10217113665389528No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA40.10217113665389528No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA40.10217113665389528No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG40.10217113665389528No Hit
CCTATTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATAGCCCAGCA40.10217113665389528No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA40.10217113665389528No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATTTCGGTG40.10217113665389528No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG40.10217113665389528No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGAG40.10217113665389528No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG40.10217113665389528No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA40.10217113665389528No Hit
CNTGTTCGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTTATAGTCCAGCA40.10217113665389528No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA40.10217113665389528No Hit
CNTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA40.10217113665389528No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA40.10217113665389528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)