FastQCFastQC Report
Tue 30 May 2023
EGAF00006172044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991
Sequences flagged as poor quality0
Sequence length200
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA822.7415580073553993No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT531.7719826145101973No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT501.671681711802073No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA491.6382480775660315No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT471.5713808090939485No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT441.4710799063858242No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA441.4710799063858242No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT411.3707790036776997No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA411.3707790036776997No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA351.170177198261451No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT331.103309929789368No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT331.103309929789368No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA321.0698762955533265No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT321.0698762955533265No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT311.0364426613172852No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT301.0030090270812437No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT301.0030090270812437No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA301.0030090270812437No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT290.9695753928452023No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGTGTA290.9695753928452023No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT280.9361417586091608No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA280.9361417586091608No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA260.8692744901370779No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT260.8692744901370779No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT250.8358408559010365No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC230.7689735874289535No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC220.7355399531929121No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT220.7355399531929121No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA210.7021063189568706No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA200.6686726847208292No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC190.6352390504847877No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA180.6018054162487462No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA170.5683717820127048No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT170.5683717820127048No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA160.5349381477766633No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA150.5015045135406219No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC130.43463724506853896No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC130.43463724506853896No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC120.4012036108324975No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC120.4012036108324975No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA120.4012036108324975No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC110.36776997659645605No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA110.36776997659645605No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT110.36776997659645605No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA100.3343363423604146No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC100.3343363423604146No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGAAACCCCAGTAGTCCGGC100.3343363423604146No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA100.3343363423604146No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC90.3009027081243731No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC90.3009027081243731No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA90.3009027081243731No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC90.3009027081243731No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC90.3009027081243731No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA80.26746907388833163No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC80.26746907388833163No Hit
CATGATTAGAAACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT80.26746907388833163No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA70.2340354396522902No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGATACCCTGGTAGTCCGGC70.2340354396522902No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC70.2340354396522902No Hit
CATGATTAGAAACCCTGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT70.2340354396522902No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA60.20060180541624875No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC60.20060180541624875No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGAAACCCTAGTAGTCCGGC50.1671681711802073No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGAAACCCGAGTAGTCCGGC50.1671681711802073No Hit
CATGATTAGAAACCCCAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT50.1671681711802073No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGATACCCGAGTAGTCCGGC50.1671681711802073No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGATACCCCAGTAGTCCGGC50.1671681711802073No Hit
CATGATTAGATACCCGAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT40.13373453694416582No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA40.13373453694416582No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC40.13373453694416582No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA40.13373453694416582No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGATACCCCGGTAGTCCGGC40.13373453694416582No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAA40.13373453694416582No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACATC40.13373453694416582No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGAAACCCCGGTAGTCCGGC40.13373453694416582No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGAAACCCGGGTAGTCCGGC40.13373453694416582No Hit
CATGATTAGATACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT40.13373453694416582No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGAAACCCCTGTAGTCCGGC30.10030090270812438No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAACCTC30.10030090270812438No Hit
CATGATTAGAAACCCGAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAA30.10030090270812438No Hit
CATTATAGAAACCCGTGTAGTCCGGCTGACTGACTCCAAGTCTTACAATC30.10030090270812438No Hit
AAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTGTTCCA30.10030090270812438No Hit
TTAGATACCCCGGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTCGT30.10030090270812438No Hit
AACCACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGG30.10030090270812438No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA30.10030090270812438No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAA30.10030090270812438No Hit
CTCCAAATGAAGAAATAACATCATGGTAACGCTGCATGAAGTAATCACGT30.10030090270812438No Hit
CATGATTAGATACCCTAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT30.10030090270812438No Hit
CATGATTAGAAACCCGTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT30.10030090270812438No Hit
CGGCAGCGGTCACAGGACGCATAAGCGATTAGAAACCCTAGTAGTCCGGC30.10030090270812438No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTCCAAGTCTTACAATCTC30.10030090270812438No Hit
CATGATTAGATACCCGTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT30.10030090270812438No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA30.10030090270812438No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA30.10030090270812438No Hit
CATGATTAGATACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT30.10030090270812438No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA30.10030090270812438No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTCCAAGTCTTACAAA30.10030090270812438No Hit
CGGCAGCGGTCACAGGACGCATAAGCTATTAGATACCCCTGTAGTCCGGC30.10030090270812438No Hit
CATGATTAGAAACCCCTGTAGTCCGGCTGACTGACTCCAAGTCTTACAAT30.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)