FastQCFastQC Report
Tue 30 May 2023
EGAF00006172045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991
Sequences flagged as poor quality0
Sequence length200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG2107.021063189568706No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG1956.519558676028084No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC862.8752925442995654No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC722.4072216649949847No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG441.4710799063858242No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA361.2036108324974923No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA351.170177198261451No Hit
ANTGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG341.1367435640254095No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA331.103309929789368No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA321.0698762955533265No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAATACCATAGTA301.0030090270812437No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA280.9361417586091608No Hit
CATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG270.9027081243731194No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATGGTCCAGTA270.9027081243731194No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT240.802407221664995No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA230.7689735874289535No Hit
CATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG220.7355399531929121No Hit
AGCTTATGCGTCCTGTGACCGCTGCCGTTACCGCGGCTGCTGGCACACAA210.7021063189568706No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG190.6352390504847877No Hit
AGCTTATGCGTCCTGTGACCGCTGCCGTTACCGCGGCGGCTGGCACACAA190.6352390504847877No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA190.6352390504847877No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA180.6018054162487462No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA150.5015045135406219No Hit
GNTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC140.4680708793045804No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT130.43463724506853896No Hit
AATGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG120.4012036108324975No Hit
GNTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC110.36776997659645605No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA100.3343363423604146No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTAGTGGCCCAGAG100.3343363423604146No Hit
AATGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG100.3343363423604146No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA100.3343363423604146No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA100.3343363423604146No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG90.3009027081243731No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG80.26746907388833163No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA80.26746907388833163No Hit
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG80.26746907388833163No Hit
CGCTTATGCGTCCTGTGACCGCTGCCGTTACCGCGGCGGCTGGCACACAA80.26746907388833163No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA70.2340354396522902No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC70.2340354396522902Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA70.2340354396522902No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT70.2340354396522902No Hit
AATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG70.2340354396522902No Hit
CNTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG60.20060180541624875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA60.20060180541624875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA60.20060180541624875No Hit
ATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG60.20060180541624875No Hit
GGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC60.20060180541624875No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA50.1671681711802073No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATTTCGGTG50.1671681711802073No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGAG50.1671681711802073No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG50.1671681711802073No Hit
TACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC50.1671681711802073Illumina Single End PCR Primer 1 (100% over 21bp)
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGAG50.1671681711802073No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATTTCGGTG50.1671681711802073No Hit
CNTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA40.13373453694416582No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG40.13373453694416582No Hit
CNTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA40.13373453694416582No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG40.13373453694416582No Hit
GGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC40.13373453694416582No Hit
ATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAA40.13373453694416582No Hit
CGCTTATGCGTCCTGTGACCGCTGCCGTTACCGCGGCTGCTGGCACACAA40.13373453694416582No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA30.10030090270812438No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTTGGTG30.10030090270812438No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA30.10030090270812438No Hit
CNTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAATACCATAGTA30.10030090270812438No Hit
ANCTTATGCGTCCTGTGACCGCTGCCGTTACCGCGGCGGCTGGCACACAA30.10030090270812438No Hit
ANCTTATGCGTCCTGTGACCGCTGCCGTTACCGCGGCTGCTGGCACACAA30.10030090270812438No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA30.10030090270812438No Hit
CTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG30.10030090270812438No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA30.10030090270812438No Hit
CATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG30.10030090270812438No Hit
CNCTTATGCGTCCTGTGACCGCTGCCGTTACCGCGGCGGCTGGCACACAA30.10030090270812438No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG30.10030090270812438No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA30.10030090270812438No Hit
CNTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATGGTCCAGTA30.10030090270812438No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA30.10030090270812438No Hit
TNTGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG30.10030090270812438No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG30.10030090270812438No Hit
TGTGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG30.10030090270812438No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATTTCGGTGGTC30.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)