FastQCFastQC Report
Sun 21 May 2023
EGAF00006172054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3501
Sequences flagged as poor quality0
Sequence length200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA38711.053984575835475No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA802.2850614110254215No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA732.085118537560697No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT691.970865467009426No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT641.828049128820337No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT631.7994858611825193No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA621.7709225935447013No Hit
CATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC551.570979720079977No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA531.5138531848043417No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA501.4281633818908883No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA501.4281633818908883No Hit
CATTAGAAACCCTGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC491.3996001142530705No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT451.2853470437017995No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA401.1425307055127107No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA401.1425307055127107No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA381.0854041702370751No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT371.0568409025992573No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT371.0568409025992573No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA371.0568409025992573No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC371.0568409025992573No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA361.0282776349614395No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA361.0282776349614395No Hit
CATTATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT350.9997143673236218No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT340.971151099685804No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA340.971151099685804No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT330.9425878320479864No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT300.8568980291345331No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA300.8568980291345331No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT280.7997714938588976No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC280.7997714938588976No Hit
CATTAGAAACCCGTGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC270.7712082262210797No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT260.742644958583262No Hit
CATTAGAAACCCTTGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC260.742644958583262No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT240.6855184233076265No Hit
CATTAGATACCCTGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC240.6855184233076265No Hit
CATTAGAAACCCTAGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC230.6569551556698086No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA220.6283918880319909No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT220.6283918880319909No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTACGTA200.5712653527563554No Hit
CATTAGAAACCCGAGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC200.5712653527563554No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA200.5712653527563554No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA200.5712653527563554No Hit
CATTAGATACCCTTGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC190.5427020851185376No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGTGTGTA190.5427020851185376No Hit
CATTAGATACCCCGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC170.485575549842902No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA170.485575549842902No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT170.485575549842902No Hit
CATTAGAAACCCCAGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC170.485575549842902No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC160.4570122822050843No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA160.4570122822050843No Hit
CATTAGATACCCTAGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC160.4570122822050843No Hit
TACGTAGGATCCGAGCGTTATCCGGAGTGACTGGGCGTAAAGAGTTGCGT140.3998857469294488No Hit
CATTAGATACCCCTGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC140.3998857469294488No Hit
CATTAGATACCCGTGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC140.3998857469294488No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT130.371322479291631No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA130.371322479291631No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA100.2856326763781777No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT100.2856326763781777No Hit
CATTAGATACCCCAGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC100.2856326763781777No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA100.2856326763781777No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA90.2570694087403599No Hit
CATTAGATACCCGAGTAGTCCGGCTGACTGACTAGATGTTCTGCTATCTC90.2570694087403599No Hit
TACGGAGGGTGCAAGTGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA80.22850614110254214No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTAGATGTTCTGCTAT70.1999428734647244No Hit
ACAATTAGAAACCCTGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATC70.1999428734647244No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA60.17137960582690662No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA60.17137960582690662No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGCTTAAAGGGTGCGTA60.17137960582690662No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA50.14281633818908884No Hit
TACGAGGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA50.14281633818908884No Hit
ACAATTAGAAACCCCGGTAGTCCGGCTGACTGACTAGATGTTCTGCTATC40.11425307055127107No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA40.11425307055127107No Hit
GACGTAGGATGCAAGCGTTGTCCGGATTCATTGGGCGTAAAGAGTTCGTA40.11425307055127107No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA40.11425307055127107No Hit
CATTAGAAACCCGGGTAGTCCGGCTGACTGACTAGATGTTCTGCTACCTC40.11425307055127107No Hit
CATTAGAAACCCCTGTAGTCCGGCTGACTGACTAGATGTTCTGCTACCTC40.11425307055127107No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGCA40.11425307055127107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)