FastQCFastQC Report
Tue 30 May 2023
EGAF00006172055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3501
Sequences flagged as poor quality0
Sequence length200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC2828.05484147386461No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG2256.426735218508997No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG2065.88403313339046No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC1795.11282490716938No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC1283.656098257640674No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA541.5424164524421593No Hit
CNTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC491.3996001142530705No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA461.313910311339617No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG411.1710939731505283No Hit
ANTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG350.9997143673236218No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCGTAGTA320.9140245644101685No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA320.9140245644101685No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG310.8854612967723506No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA300.8568980291345331No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA290.8283347614967151No Hit
GNTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC290.8283347614967151No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG260.742644958583262No Hit
GNTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC260.742644958583262No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA260.742644958583262No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTAAATCTTAGTG240.6855184233076265No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA230.6569551556698086No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG210.5998286203941731No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTC200.5712653527563554No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT190.5427020851185376No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTATCTGTCCAGTT170.485575549842902No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAACAGTCGTCCAGTG170.485575549842902No Hit
CCTGTTTGCTCCCCACACTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG170.485575549842902No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG160.4570122822050843No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTT150.42844901456726653No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA150.42844901456726653No Hit
ANTGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG150.42844901456726653No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA150.42844901456726653No Hit
AATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG140.3998857469294488No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG140.3998857469294488No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG140.3998857469294488No Hit
AATGGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG130.371322479291631No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA120.34275921165381323No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG120.34275921165381323No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG120.34275921165381323No Hit
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG120.34275921165381323No Hit
AATGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG110.31419594401599543No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA110.31419594401599543No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA90.2570694087403599No Hit
TGTGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG90.2570694087403599No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA80.22850614110254214No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA80.22850614110254214No Hit
CNTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG80.22850614110254214No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA80.22850614110254214No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA80.22850614110254214No Hit
CNTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG80.22850614110254214No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA80.22850614110254214No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGTCTAGTC70.1999428734647244No Hit
GGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC70.1999428734647244No Hit
GTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC70.1999428734647244No Hit
TGTGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG70.1999428734647244No Hit
GGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC70.1999428734647244No Hit
CNTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG70.1999428734647244No Hit
CCCATTTGCTCCCCTAGCTTTCGTTCCTCAGCGTCAGTTACAGCCCAGCA60.17137960582690662No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA60.17137960582690662No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCTGTCTAGCC50.14281633818908884No Hit
ATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG50.14281633818908884No Hit
CNTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG50.14281633818908884No Hit
CNTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTC50.14281633818908884No Hit
CNTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA50.14281633818908884No Hit
CNGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGTCTAGTC50.14281633818908884No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG50.14281633818908884No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC50.14281633818908884Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTACATCTTAGTG50.14281633818908884No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCTGTGTAGCC50.14281633818908884No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG40.11425307055127107No Hit
CNTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTAAATCTTAGTG40.11425307055127107No Hit
CNTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCGTAGTA40.11425307055127107No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTCTAGCC40.11425307055127107No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA40.11425307055127107No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTT40.11425307055127107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)