FastQCFastQC Report
Tue 30 May 2023
EGAF00006172056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4946
Sequences flagged as poor quality0
Sequence length200
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1152.325111200970481No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1002.0218358269308534No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA661.334411645774363No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA531.0715729882733522No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA440.8896077638495753No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA430.8693894055802669No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA330.6672058228871816No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA330.6672058228871816No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA310.6267691063485645No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA310.6267691063485645No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA230.4650222401940962No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA220.44480388192478765No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA220.44480388192478765No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA220.44480388192478765No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA170.34371209057824503No Hit
CATTATTAGAAACCCTAGTAGTCCGGCTGACTGACTCCACCTACTCCAAT160.3234937323089365No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA160.3234937323089365No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA140.28305701577031944No Hit
CATTATTAGATACCCTAGTAGTCCGGCTGACTGACTCCACCTACTCCAAT130.26283865750101093No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA120.2426202992317024No Hit
CATTATTAGAAACCCCTGTAGTCCGGCTGACTGACTCCACCTACTCCAAT110.22240194096239382No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA100.20218358269308534No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA100.20218358269308534No Hit
CATTATTAGATACCCGAGTAGTCCGGCTGACTGACTCCACCTACTCCAAT100.20218358269308534No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA100.20218358269308534No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA100.20218358269308534No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA100.20218358269308534No Hit
CATTATTAGATACCCCTGTAGTCCGGCTGACTGACTCCACCTACTCCAAT90.1819652244237768No Hit
CATTATTAGAAACCCGAGTAGTCCGGCTGACTGACTCCACCTACTCCAAT90.1819652244237768No Hit
CATTATTAGAAACCCTTGTAGTCCGGCTGACTGACTCCACCTACTCCAAT90.1819652244237768No Hit
CATTATTAGATACCCGTGTAGTCCGGCTGACTGACTCCACCTACTCCAAT90.1819652244237768No Hit
CATTATTAGAAACCCTGGTAGTCCGGCTGACTGACTCCACCTACTCCAAT80.16174686615446826No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA80.16174686615446826No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA70.14152850788515972No Hit
CATTATTAGATACCCCGGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
CATTATTAGATACCCTTGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA70.14152850788515972No Hit
CATTATTAGAAACCCGGGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
CATTATTAGATACCCCAGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA70.14152850788515972No Hit
CATTATTAGATACCCGGGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA70.14152850788515972No Hit
CATTATTAGAAACCCGTGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
CATTATTAGAAACCCCGGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
CATTATTAGATACCCTGGTAGTCCGGCTGACTGACTCCACCTACTCCAAT70.14152850788515972No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA60.1213101496158512No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA60.1213101496158512No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA60.1213101496158512No Hit
TACGAAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA60.1213101496158512No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA60.1213101496158512No Hit
CATTAGATACCCGGGTAGTCCGGCTGACTGACTCCACCTACTCCAATCTC50.10109179134654267No Hit
TCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCGGTGGTCTATAGT50.10109179134654267No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA50.10109179134654267No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA50.10109179134654267No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA50.10109179134654267No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA50.10109179134654267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)