FastQCFastQC Report
Sun 21 May 2023
EGAF00006172154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194346
Sequences flagged as poor quality0
Sequence length200
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4044620.81133648235621No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA3035815.620594197976803No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA158968.179226739938048No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA129136.644335360645447No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA70383.621376308233769No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA63383.261193953052803No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA56392.901526144093524No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA55492.8552169841416855No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGAGGGAGCA49352.53928560402581No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA26061.3409074537165673No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA24251.2477745875912034No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA23761.2225618227285358No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA22361.1505253516923426No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA21271.0944398135284492No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA21171.0892943513115783No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20421.0507033846850462No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA18620.9580850647813693No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA11000.5660008438558035No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGTGCA10820.556739011865436No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA10760.5536517345353132No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10150.5222644150124005No Hit
TACGGAGGATCCGAGCGCTATCCGGATTTATTGGGTTTAAAGGGAGCGTA8510.43787883465571714No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA7940.4085497000195527No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA7070.3637841787327756No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA6460.33239685920986284No Hit
TACGGAGGATCCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA6410.32982412810142736No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA5790.2979222623568275No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA5300.2727094974941599No Hit
TACGGAGGATGCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA4600.2366912619760633No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4210.21662395933026665No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGTACGTA4200.21610941310857956No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4110.2114784971133957No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3890.20015848023627963No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA3780.1944984717977216No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA3640.18729482469410227No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGTA3430.1764893540386733No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA3340.17185843804348946No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA3310.17031479937842817No Hit
TACGTATGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA3250.1672275220483056No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA3140.16156751360974755No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA3010.15487841272781533No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA2920.1502474967326315No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGCA2840.14613112695913474No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA2550.13120928653020902No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGCA2540.13069474030852193No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGCA2500.12863655542177355No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA2490.12812200920008643No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2390.1229765469832155No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA2360.12143290831815423No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA2290.11783108476634456No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGCGCGCGCA2250.11577289987959617No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA2220.1142292612145349No Hit
TACGTAGGGAGCAAGTGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA2220.1142292612145349No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGCA2000.10290924433741884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)