FastQCFastQC Report
Sun 21 May 2023
EGAF00006172569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTATCGTATTAGTA1556.378600823045268No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGG1134.650205761316872No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG1014.156378600823045No Hit
CCTGTTTGCTCCCCATGCTTTCGCGTCTCAGCGTCAGTCACTGTCCAGAG863.539094650205761No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG803.292181069958848No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTAAGACTTAGTA722.9629629629629632No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG652.674897119341564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCGGACCAGTG642.633744855967078No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG572.345679012345679No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA532.1810699588477367No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTG522.139917695473251No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAAAGGACCAGGT492.016460905349794No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG441.8106995884773662No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGACAAGGCCCAGTG441.8106995884773662No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCTGTCCAGGT411.6872427983539096No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG361.4814814814814816No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGCCCAGGT351.440329218106996No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG341.3991769547325104No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC311.2757201646090535No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTTGATCCAGAT301.2345679012345678No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA301.2345679012345678No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATTTGTGTAGCA291.1934156378600824No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA271.1111111111111112No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC271.1111111111111112No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAG261.0699588477366255No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCATGGCCCAGAA261.0699588477366255No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA210.8641975308641975No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA200.823045267489712No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGCCCAGTA200.823045267489712No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA190.7818930041152263No Hit
CCTGTTTGATCCCCACGCTATCGTGCATGAGCGTCAGAAACGGGCCAGTG170.6995884773662552No Hit
CCTGTTTGCTCCCCACGCCTTCGTGCCTCAGCGTCAGTGTTGATCCAGAT160.6584362139917695No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG150.6172839506172839No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG140.5761316872427984No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTAACAGCCCAGAG140.5761316872427984No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGTC140.5761316872427984No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT130.5349794238683128No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA120.49382716049382713No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG110.45267489711934156No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA100.411522633744856No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTGAGCGTCAGTCTTCGTCCAGGG100.411522633744856No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG80.3292181069958848No Hit
CCTGTTTGATCCCCTTGCTCTCGCGCCTCAGCGTCAGTTATTAGCTAGAG80.3292181069958848No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGTG70.2880658436213992No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACACCTTAGTA70.2880658436213992No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGACCAGGA70.2880658436213992No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGGG60.24691358024691357No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT60.24691358024691357No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA60.24691358024691357No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTAAGCCTTAGTA60.24691358024691357No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAGCGCGCTGGAA60.24691358024691357No Hit
ATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG60.24691358024691357No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGCCAAGGCCCAGTG60.24691358024691357No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTCAGTGTCAGTATTAGCCCAGGT60.24691358024691357No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA60.24691358024691357No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG50.205761316872428No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA50.205761316872428No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA50.205761316872428No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG50.205761316872428No Hit
AATGATACCGCGGCTGCTGGCACACCATTACCATAGTGTAGATCTCGGTG50.205761316872428No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA40.1646090534979424No Hit
CCCGTTCGATTCCCACGCTTTCGTGTTTCAGCGTCAGGTACGCGCCAGTA40.1646090534979424No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCCTCAGCGTCAGTCACTGTCCAGAG40.1646090534979424No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCTAGAA40.1646090534979424No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG40.1646090534979424No Hit
AATGATACCGCGGCGGCTGGCACACCATTACCATAGTGTAGATCTCGGTG40.1646090534979424No Hit
ACTGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG40.1646090534979424No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA30.12345679012345678No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGATGCCGGCCCAGGA30.12345679012345678No Hit
GATACCGCTGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC30.12345679012345678Illumina Single End PCR Primer 1 (95% over 21bp)
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGTCCCAGGG30.12345679012345678No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG30.12345679012345678No Hit
CCTGTTTGCTCCCCACTCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG30.12345679012345678No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCCTAGTGTAGATCTCGGTG30.12345679012345678No Hit
CCTGTTTGCTCCCCACGCTTTCGTCCCTCAGTGTCAGTTACAGCCCAGTA30.12345679012345678No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGTG30.12345679012345678No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC30.12345679012345678Illumina Single End PCR Primer 1 (100% over 21bp)
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTAGGTG30.12345679012345678No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTTGCTCCAGAT30.12345679012345678No Hit
CCTGTTTGATCCCCACGCTCTCGTGCATGAGCGTCAGAAACGGGCCAGTG30.12345679012345678No Hit
GGTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC30.12345679012345678No Hit
AATGGTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG30.12345679012345678No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA30.12345679012345678No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATTAGCGTCAGTGTTGGCCCAGGG30.12345679012345678No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT30.12345679012345678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)