FastQCFastQC Report
Tue 30 May 2023
EGAF00006172571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121
Sequences flagged as poor quality0
Sequence length200
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG716.333630686886709No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA554.906333630686887No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTATCGTATTAGTA454.014272970561998No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA322.8545941123996434No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC302.6761819803746656No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG272.408563782337199No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG242.140945584299732No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG232.0517395182872438No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA221.9625334522747548No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG211.873327386262266No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG171.5165031222123104No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC161.4272970561998217No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC161.4272970561998217No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA141.2488849241748439No Hit
AATGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG110.9812667261373774No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGG100.8920606601248885No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA80.7136485280999109No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT80.7136485280999109No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATCGACCCAGGG80.7136485280999109No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG70.6244424620874219No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG70.6244424620874219No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA70.6244424620874219No Hit
AATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG70.6244424620874219No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC50.44603033006244425No Hit
CCTATTTGCTCCCCACGCTTTCGGGCATGAGCGTCAGTTGCGGCCCAGAC50.44603033006244425No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCATCGTCAGTATCGTATTAGTA50.44603033006244425No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA50.44603033006244425No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA50.44603033006244425No Hit
CCTGTTTGATCCCCACGCTTTCGAGCTATAGCGTCAGTACTGGACCAGGT40.35682426404995543No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTTGATCCAGAT40.35682426404995543No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTTATAGTTTCGTT40.35682426404995543No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA40.35682426404995543No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGTCGGACCAGGA40.35682426404995543No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATATCGTATTAGTA40.35682426404995543No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA40.35682426404995543No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATCGTGTAGATCTCGGTG40.35682426404995543No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA30.2676181980374665No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA30.2676181980374665No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA30.2676181980374665No Hit
ATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG30.2676181980374665No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC30.2676181980374665No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA30.2676181980374665No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA30.2676181980374665No Hit
CCTGTTCGCTACCCATGCTCTCGCTCCTCAGCGTCAGTAACTGCCCAGAG30.2676181980374665No Hit
AATGATACCGCGGCGGCTGGCACACCATTACCATAGTGTAGATCTCGGTG30.2676181980374665No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTGCAGTCCAGTA30.2676181980374665No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGATGATGCCCAGCT30.2676181980374665No Hit
CCTGTTCGCTCCCCACACTTTCGCTCCTCAGCGTCAGGTCATGCCCAGAG30.2676181980374665No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA30.2676181980374665No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAATGGCCAGAA30.2676181980374665No Hit
GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAAT20.17841213202497772No Hit
ATTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC20.17841213202497772No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG20.17841213202497772No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGGTATCGTCCAGTA20.17841213202497772No Hit
ACTGTTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.17841213202497772No Hit
CCTGTTCGCTCCCCAAGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG20.17841213202497772No Hit
ATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGG20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG20.17841213202497772No Hit
ATTACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCGACGTCAGTTACCGTCCAGTA20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG20.17841213202497772No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGGA20.17841213202497772No Hit
AATGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTAGGTG20.17841213202497772No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA20.17841213202497772No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGAG20.17841213202497772No Hit
TTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATA20.17841213202497772No Hit
ACTGTTACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG20.17841213202497772No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCTGTGGTC20.17841213202497772No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGTCCAGTG20.17841213202497772No Hit
TCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG20.17841213202497772No Hit
CCCTTTCGCTCCCCTGGCTTTCGTCCATGAGCGTCAGTTATGGCCCAGCA20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTAAAGTCCAGAG20.17841213202497772No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG20.17841213202497772No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA20.17841213202497772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)