FastQCFastQC Report
Sun 21 May 2023
EGAF00006172573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3767
Sequences flagged as poor quality0
Sequence length200
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC70618.741704273958057No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGGCCAGTG39410.459251393681974No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1724.565967613485532No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGGGCCAGTG1594.2208654101406955No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1453.8492168834616405No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCGTAGTA1143.0262808601008757No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG1052.787363950092912No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG842.2298911600743296No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA782.0706132200690206No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC721.9113352800637111No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA701.8582426333952746No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTATCGTATTAGTA611.6193257233873108No Hit
CCCATTTGCTCCCCTAGCTTTCGTTCCTCAGCGTCAGTTACAGCCCAGCA551.4600477833820016No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCACGTCCCAGGG501.3273161667109106No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGACCAGTT401.0618529333687283No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAT381.008760286700292No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA320.8494823466949828No Hit
CCTATTTGCTCCCCACGCTTTCGGGCATGAGCGTCAGTTGCGGCCCAGAC200.5309264666843642No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCACTATATGTGTAGCC180.4778338200159278No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA160.4247411733474914No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTGAGCGTCAGTCTTTGTCCAGGG130.34510220334483677No Hit
CCCTTTTGCTCCCCAAGCTTTCGTCCATGAGCGTCAGTCGTGTCCTAGAA100.2654632333421821No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG90.2389169100079639No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTCAGCGTCAGTATCGGACCAGTT70.18582426333952748No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG60.15927794000530926No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG60.15927794000530926No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA60.15927794000530926No Hit
CCTGTTTGATCCCCACGCCTTCGTGCCTCAGCGTCAATATATGTGTAGCC50.13273161667109104No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG50.13273161667109104No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTCTAGCC50.13273161667109104No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTTTCGGCCCAGCA40.10618529333687285No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA40.10618529333687285No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCTGTGTAGCC40.10618529333687285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)