FastQCFastQC Report
Wed 31 May 2023
EGAF00006172581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106388
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2693925.321464826860172No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA101379.528330262811595No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA81657.674737752378087No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG73696.926533067639208No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT57195.37560627138399No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC41473.897996014588111No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA21882.056622927397827No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA18191.7097792984171147No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG17001.5979245779599205No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA15461.4531714103094335No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA15011.4108734067752002No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG14421.3554160243636502No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA12651.1890438771289995No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA10971.0311313306011956No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG10230.9615746136782344No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG7580.7124863706433057No Hit
CCTATTTGCTCCCCACGCTTTCGGGCATGAGCGTCAGTTGCGGCCCAGAC7440.6993269917659887No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA7410.6965071248637065No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA6570.6175508515998045No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6570.6175508515998045No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA6490.6100312065270519No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA5820.5470541790427491No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGTATCCCAGGG5790.544234312140467No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA5010.47091777268112944No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG4820.45305861563334204No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA4540.4267398578787081No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA4240.39854118885588596No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA3770.3543632740534647No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA3540.33274429446930104No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA3360.31582509305560774No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3260.3064255367146671No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT3180.2989058916419145No Hit
CCTGTTCGATACCCTCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG3140.2951460691055382No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA2500.2349889085235177No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGATGCCGGCCCAGGA2170.20397037259841333No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2100.1973906831597549No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTGAGCGTCAGTCTTTGTCCAGGG2090.19645072752566078No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1610.1513328570891454No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG1460.13723352257773433No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1410.13253374440726398No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT1320.12407414370041733No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1290.12125427679813514No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG1280.12031432116404106No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCACTTT1240.11655449862766477No Hit
CCTGTTCGATACCCGCACTTTCGAGCGTCAGCGTCAGTTGCGCTCCAGTG1220.11467458735947664No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT1220.11467458735947664No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAACAGGCAGAGCAGA1180.11091476482310036No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1160.10903485355491221No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA1120.10527503101853593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)