FastQCFastQC Report
Sun 21 May 2023
EGAF00006172638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116302
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA3574730.7363587900466No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2015117.326443225395952No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT88967.649051607023096No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGGGCGTAAAGCGCTCGTA48594.177916114942133No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA42793.6792144589087035No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA16581.4255988719024608No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA14181.2192395659575932No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA13381.1504531306426373No Hit
TACGTAGGGTACGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA13381.1504531306426373No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA11440.9836460250038693No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA11410.9810665336795584No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA11320.9733280597066258No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA10950.9415143333734588No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA8110.6973224880053653No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGAGCGTAAAGAGCTCGTA7000.6018813090058641No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA6390.5494316520782101No Hit
TACGTAGGGTCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA5790.4978418255919933No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCTTAAAGAGTGTTTTAGATCA5640.484944368970439No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA5030.43249471204278517No Hit
TACGGAGGGTGCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA4940.42475623806985263No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA4400.3783253942322574No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4270.36714759849357703No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA4110.35339031143058586No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA2950.25364998022389984No Hit
GTTTTAATTTTTGTAGCGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT2790.23989269316090867No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2670.22957472786366528No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2590.22269608433216967No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTACAGAGTGTTTTAGATCA2510.21581744080067408No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA2470.21237811903492632No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA2470.21237811903492632No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCATAAAGAGTGTTTTAGATCA2360.20291998417911986No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2330.20034049285480904No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCGTAAAGAGTGTTTTAGATCA2300.1977610015304982No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCA2190.18830286667469176No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2160.18572337535038091No Hit
GTTTTAATTTTTGTCGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT1960.16852676652164195No Hit
CACGATTAACCCTAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1950.167666936080205No Hit
GTTTTAATTTTTGTAGAGAAGTAGTCTTGCTATGTTGCCCAGGCTGGTCT1930.16594727519733107No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTCTGTTGCCCAGGCTGGTCT1910.1642276143144572No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC1870.1607882925487094No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTTTTTTAGATCA1810.1556293099000877No Hit
TACGTAGGGTACGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTCGTA1740.14961049681002905No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCTCGTA1700.14617117504428126No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA1690.1453113446028443No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATATTGCCCAGGCTGGTCT1590.13671304018847483No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAGAGAGTGTTTTAGATCA1550.13327371842272703No Hit
CACGATTAACCCAAGTCCATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1540.13241388798129008No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1530.13155405753985314No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1510.12983439665697924No Hit
CACGATTAACCCAAGTCAATATAAGCCGGCGTAAAGAGTGTTTTAGATCA1490.12811473577410534No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCGTAAAGAGTGTTTTAGATCA1460.1255352444497945No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTAGGCGTAAAGAGCTCGTA1420.12209592268404669No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGTGTA1370.11779677047686196No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA1310.11263778782824027No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA1290.11091812694536637No Hit
TACGTAGGGTGCGAGCGTTATCCGGATTTATTGGGCGTAAAGAGCTCGTA1280.11005829650392943No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA1250.10747880517961858No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAATAGTGTTTTAGATCA1240.10661897473818163No Hit
TACGTATGTTCCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1230.10575914429674468No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1220.10489931385530772No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTCAAGAGTGTTTTAGATCA1210.10403948341387079No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1210.10403948341387079No Hit
TACAGAGGTCCCGAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA1180.10145999208955993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)