FastQCFastQC Report
Sun 21 May 2023
EGAF00006172657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102596
Sequences flagged as poor quality0
Sequence length200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT2565125.00194939373855No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC77737.576318764864127No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA40373.934851261257749No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCGGGCCAGTG30692.9913446918008497No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA29272.852937736363991No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA29132.8392919801941594No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG25122.4484385356154235No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20812.028344184958478No Hit
CCCATTTGCTCCCCTAGCTTTCGTTCCTCAGCGTCAGTTACAGCCCAGCA17911.7456820928691177No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA10271.0010136847440445No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG9570.9327849038948887No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGGGCCAGTG9250.9015946040781316No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA7540.7349214394323366No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA7380.719326289523958No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTT7170.6988576552692113No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG6930.6754649304066435No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT6800.662793871106086No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT6700.6530469024133494No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT6660.6491481149362548No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA5840.569222971655815No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA5620.5477796405317946No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA5590.5448555499239737No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG5550.5409567624468791No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCACTTT5390.5253616125385006No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT5380.5243869156692269No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG5320.5185387344535849No Hit
CCCAGTTTTGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT5290.5156146438457639No Hit
CCCAGTTTGGGTCTTAGTTATTCTGTGTTCAGATGCATTAAAGCCACTTT4860.4737026784669968No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA4760.4639557097742602No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG4530.44153768178096614No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGCTATGTTAAAGCCACTTT4480.43666419743459783No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCCGTCCAGAT4410.4298413193496823No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA4400.4288666224804086No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCCCTTT4010.390853444578736No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3920.3820811727552731No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3630.35381496354633707No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3360.32749814807594835No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG3220.3138523919061172No Hit
CCCAGTTTGGTTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT3210.31287769503684354No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA3210.31287769503684354No Hit
CCCATTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT3070.2992319388670124No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTATCTGTCCAGTT2770.2699910327888027No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATGCGTTAAAGCCACTTT2720.2651175484424344No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCACTATATGTGTAGCC2680.2612187609653398No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA2590.2524464891418769No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2440.23782603610277203No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA2430.2368513392334984No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGAGCCAGTG2160.2105345237631097No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA2100.20468634254746776No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2080.20273694880892043No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT2060.2007875550703731No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGAGCCAGTG2020.1968887675932785No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAATTCTAGACCAGTA1950.1900658895083629No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1880.18324301142344732No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA1780.17349604273071076No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1740.1695972552536161No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG1710.16667316464579515No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA1620.15790089282233224No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA1610.1569261959530586No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCTTGGCCCAGAG1610.1569261959530586No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG1600.15595149908378494No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1570.15302740847596397No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1560.15205271160669032No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1500.1462045303910484No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1300.12671059300557527No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1230.11988771492065968No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1210.11793832118211235No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1160.11306483683574409No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1110.10819135248937581No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTTTTCAGATATGTTAAAGCCACTTT1100.10721665562010214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)